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Characteristics of CD11c+CD5+ chronic B-cell leukemias and the identification of novel peripheral blood B-cell subsets with chronic lymphoid leukemia immunophenotypes.
- Source :
-
Blood [Blood] 1990 Jul 01; Vol. 76 (1), pp. 123-30. - Publication Year :
- 1990
-
Abstract
- Previous studies have indicated that chronic lymphocytic leukemias (CLL) are characterized by the coexpression of CD5 and B-cell antigens, while hairy cell leukemias (HCL) typically express CD11c+CD5- B-cell immunophenotypes. In this report we describe the features of B-cell leukemias with CD11c+CD5+ immunophenotypes and the identification of novel circulating B-cell subsets defined by the expression of CD20, CD5, and CD11c antigens. Morphologic evaluation of 14CD11c+CD5+ B-cell leukemias showed that they generally had larger cellular diameters (14 to 21 microns) and lower nuclear:cytoplasm ratios than typical small lymphocyte CLL. These cases did not exhibit the well-defined nucleoli characteristic of prolymphocytic leukemia (PLL). The presenting clinical features of CD11c+CD5+ B-cell leukemias were most consistent with CLL or PLL, and none of the evaluated cases had pancytopenia, splenomegaly, and cytoplasmic villi characteristic of HCL. Examination of normal peripheral blood (n = 6) by three-color flow cytometry identified four novel B-cell subsets with the following immunophenotypes (mean percent of total CD20+ B cells +/- SE): CD20+CD5+CD11c+ (8.0 +/- 1.6); CD20+CD5-CD11c+ (12.0 +/- 2.0); CD20+CD5+CD11c- (35.0 +/- 4.9); and CD20+CD5-CD11c- (44.0 +/- 5.0). Our findings suggest that CD11c+CD5+ B-cell leukemias with atypical morphologic features represent forms of CLL or PLL rather than HCL. In addition, we have identified novel subsets of circulating B cells defined by patterns of CD20, CD5, and CD11c expression that correspond to the immunophenotypes of chronic B-cell leukemias.
- Subjects :
- Adult
Aged
Aged, 80 and over
Antigens, CD20
Antigens, Differentiation, B-Lymphocyte immunology
B-Lymphocytes cytology
B-Lymphocytes pathology
CD5 Antigens
Cell Division
Female
Humans
Integrin alphaXbeta2
Leukemia, Hairy Cell immunology
Leukemia, Hairy Cell pathology
Leukemia, Lymphocytic, Chronic, B-Cell genetics
Leukemia, Lymphocytic, Chronic, B-Cell pathology
Leukemia, Prolymphocytic immunology
Leukemia, Prolymphocytic pathology
Lymphocyte Activation immunology
Male
Middle Aged
Phenotype
Antigens, Differentiation immunology
B-Lymphocytes immunology
Leukemia, Lymphocytic, Chronic, B-Cell immunology
Receptors, Leukocyte-Adhesion immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 76
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 1694698