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HIV-1 genetic diversity in Argentina and early diagnosis of perinatal infection.
- Source :
-
Medicina [Medicina (B Aires)] 2006; Vol. 66 (4), pp. 319-26. - Publication Year :
- 2006
-
Abstract
- HIV-1 diagnosis of perinatally exposed children is usually performed by molecular biology-based methods, allowing the direct detection of the virus. Thus, HIV-1 genomic variability within and across strains plays a major role in relation to the sensitivity of these tests, often leading to misdiagnosis. We describe the performance of an in-house multiplex nested PCR (nPCR) for early detection of HIV-1 infection in perinatally exposed children born in Argentina, where the percentage of diverse BF recombinants is as high as 80%. After evaluation of 1316 HIV-1 perinatally exposed children collected over a 7-year period, the specificity and sensitivity of the diagnostic nPCR was of 100% and 99.2% respectively, with only two false negative cases indicating a good performance of the diagnostic nPCR in the Argentine pediatric cohort. In search of unusual HIV-1 subtypes among 22 HIV-1 infected cases presenting partial or complete HIV-1 gene amplification failure, we performed phylogenetic and recombination analysis of a vpu-env fragment in addition to gag and env Heteroduplex Mobility Assay screening. The most unusual findings included two subtypes A and a novel BC recombinant, while the majority of the strains were a variety of different BF recombinants. These results indicate the presence of novel and heterogeneous genotypes in our country and the need of continuous viral surveillance not only for diagnostic test optimization but also for the eventual implementation of a successful vaccine.
- Subjects :
- Argentina
Child
False Negative Reactions
Female
Genotype
HIV Infections diagnosis
HIV Infections transmission
HIV-1 isolation & purification
Heteroduplex Analysis
Humans
Infectious Disease Transmission, Vertical
Male
Perinatal Care
Retrospective Studies
Sensitivity and Specificity
Sequence Analysis, DNA
Viral Load
HIV Infections virology
HIV-1 genetics
Polymerase Chain Reaction methods
Recombination, Genetic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0025-7680
- Volume :
- 66
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Medicina
- Publication Type :
- Academic Journal
- Accession number :
- 16977967