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Parathyroid hormone-related peptide in plasma of patients with hypercalcemia and malignant lesions.
- Source :
-
Mayo Clinic proceedings [Mayo Clin Proc] 1990 Nov; Vol. 65 (11), pp. 1399-407. - Publication Year :
- 1990
-
Abstract
- We developed and validated a radioimmunoassay for circulating human parathyroid hormone-related peptide (PTHrP), based on a commercial antiserum to the synthetic 1-34 fragment of PTHrP, 125I-Tyr degrees-PTHrP(1-34) as radioligand, and prior extraction of the native peptide from plasma with C-2 cartridges. We determined immunoreactive PTHrP concentrations in plasma samples from 48 healthy persons (mean +/- SD, 3.1 +/- 1.0 pmol/liter; range, less than 2 to 5 pmol/liter), 8 patients with primary hyperparathyroidism, 36 patients with hypercalcemia and a concurrent malignant lesion, and 9 normocalcemic patients with cancer and increased serum levels of carcinoembryonic antigen or prostate-specific antigen. PTHrP was normal in samples from patients with primary hyperparathyroidism (3.2 +/- 1.1 pmol/liter), secondary hyperparathyroidism (2.5 +/- 1.3 pmol/liter), and cancer without hypercalcemia (2.4 +/- 1.0 pmol/liter). In contrast, plasma immunoreactive PTHrP levels were increased (6.0 to 85.0 pmol/liter) in 47% of patients with hypercalcemia and cancer of various types, with or without bone metastatic lesions. Large amounts of PTHrP were also found in conditioned medium from cultured human prostatic carcinoma cells. Thus, PTHrP may be a causative factor for hypercalcemia associated with a malignant lesion in at least half of the cases. Measurement of circulating PTHrP may be of differential diagnostic help in hypercalcemic states.
- Subjects :
- Adenoma, Islet Cell blood
Aged
Antigens, Neoplasm analysis
Biomarkers, Tumor analysis
Bone Neoplasms blood
Bone Neoplasms secondary
Breast Neoplasms blood
Breast Neoplasms drug therapy
Carcinoembryonic Antigen analysis
Carcinoma, Renal Cell blood
Carcinoma, Squamous Cell blood
Female
Humans
Hypercalcemia etiology
Hyperparathyroidism blood
Hyperparathyroidism etiology
Kidney Failure, Chronic blood
Kidney Failure, Chronic complications
Kidney Neoplasms blood
Lymphoma blood
Multiple Myeloma blood
Neoplasms complications
Pancreatic Neoplasms blood
Parathyroid Hormone-Related Protein
Prostate-Specific Antigen
Radioimmunoassay
Reference Values
Urinary Bladder Neoplasms blood
Hypercalcemia blood
Neoplasm Proteins blood
Neoplasms blood
Proteins analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0025-6196
- Volume :
- 65
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Mayo Clinic proceedings
- Publication Type :
- Academic Journal
- Accession number :
- 1700240
- Full Text :
- https://doi.org/10.1016/s0025-6196(12)62163-6