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Identification of lipopolysaccharide-binding proteins in porcine milk.
- Source :
-
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire [Can J Vet Res] 2006 Oct; Vol. 70 (4), pp. 243-50. - Publication Year :
- 2006
-
Abstract
- Septicemia and endotoxemia initiated by bacterial lipopolysaccharide (LPS) are relatively common in suckling and weaned piglets. Maternal milk is a source of both nutrition and immune protection for piglets. Passive transfer of colostral antibodies is necessary for protection of neonatal piglets against diseases, but the concentration of immunoglobulins in milk rapidly declines during the 1st wk of lactation in all mammals. We hypothesized, therefore, that nonimmunoglobulin substances in milk contribute to the innate protection of neonates against septicemia during the suckling period. Using LPS-affinity chromatography for isolation of LPS-binding proteins and liquid chromatography-mass spectrometry for their identification, we identified in porcine milk the following proteins with LPS-binding capacity: lactoferrin, soluble CD14, serum amyloid A, alpha-S1 casein, beta-casein, and kappa-casein. For lactoferrin, alpha-S1 casein, and kappa-casein, in vitro pepsin digestion did not inhibit LPS-binding activity, whereas combined digestion with pepsin and pancreatin abolished it. The biologic functions of these LPS-binding proteins and peptides were not determined.
- Subjects :
- Animals
Animals, Suckling immunology
Caseins immunology
Caseins isolation & purification
Chromatography, Affinity
Gas Chromatography-Mass Spectrometry
Hydrogen-Ion Concentration
Lactoferrin immunology
Lactoferrin isolation & purification
Lipopolysaccharide Receptors immunology
Lipopolysaccharide Receptors isolation & purification
Milk chemistry
Serum Amyloid A Protein immunology
Serum Amyloid A Protein isolation & purification
Acute-Phase Proteins immunology
Acute-Phase Proteins isolation & purification
Carrier Proteins immunology
Carrier Proteins isolation & purification
Escherichia coli physiology
Membrane Glycoproteins immunology
Membrane Glycoproteins isolation & purification
Milk immunology
Swine
Subjects
Details
- Language :
- English
- ISSN :
- 0830-9000
- Volume :
- 70
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
- Publication Type :
- Academic Journal
- Accession number :
- 17042375