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[Study on the effect and its mechanisms of genistein on the cell cycle of highly metastatic ovarian carcinoma HO-8910PM cells].

[Study on the effect and its mechanisms of genistein on the cell cycle of highly metastatic ovarian carcinoma HO-8910PM cells].

Authors :
Ma WL
Ding H
Fu WY
Zhou KY
Source :
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2006 Jul; Vol. 29 (7), pp. 686-9.
Publication Year :
2006

Abstract

Objective: To study the effect and its possible mechanism of genistein on the cell cycle of human highly metastatic ovarian carcinoma HO-8910PM cells.<br />Methods: Trypan blue stain assay was used to examine the effect of genistein on proliferation of HO-8910PM cells after 24 hours treatment. The cell cycle was assessed by flowcytometry (FCM). The expression level of NF-kappaB (p65) and the level of VEGF were assessed by Western blot analysis.<br />Results: Genistein could inhibit the proliferation of HO-8910PM cell and block the cell cycle at G1 phase. The expession level of NF-kappaB (P65) protein decreased obviously in HO-8910PM cells treated with 25 approximately 100 micromol/L genistein for 24 hours, and the effect appeared in the experssion of VEGF.<br />Conclusion: The effect on cell cycle of genistein is involved in the decreasing expression of NF kappaB (p65) and the level of VEGF.

Details

Language :
Chinese
ISSN :
1001-4454
Volume :
29
Issue :
7
Database :
MEDLINE
Journal :
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
Publication Type :
Academic Journal
Accession number :
17059009