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[Study on the effect and its mechanisms of genistein on the cell cycle of highly metastatic ovarian carcinoma HO-8910PM cells].
[Study on the effect and its mechanisms of genistein on the cell cycle of highly metastatic ovarian carcinoma HO-8910PM cells].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2006 Jul; Vol. 29 (7), pp. 686-9. - Publication Year :
- 2006
-
Abstract
- Objective: To study the effect and its possible mechanism of genistein on the cell cycle of human highly metastatic ovarian carcinoma HO-8910PM cells.<br />Methods: Trypan blue stain assay was used to examine the effect of genistein on proliferation of HO-8910PM cells after 24 hours treatment. The cell cycle was assessed by flowcytometry (FCM). The expression level of NF-kappaB (p65) and the level of VEGF were assessed by Western blot analysis.<br />Results: Genistein could inhibit the proliferation of HO-8910PM cell and block the cell cycle at G1 phase. The expession level of NF-kappaB (P65) protein decreased obviously in HO-8910PM cells treated with 25 approximately 100 micromol/L genistein for 24 hours, and the effect appeared in the experssion of VEGF.<br />Conclusion: The effect on cell cycle of genistein is involved in the decreasing expression of NF kappaB (p65) and the level of VEGF.
- Subjects :
- Cell Proliferation drug effects
Down-Regulation
Female
Flow Cytometry
Genistein administration & dosage
Humans
NF-kappa B biosynthesis
Neoplasm Metastasis
Ovarian Neoplasms metabolism
Plants, Medicinal chemistry
Tumor Cells, Cultured
Vascular Endothelial Growth Factor A biosynthesis
Antineoplastic Agents, Phytogenic pharmacology
Cell Cycle drug effects
Genistein pharmacology
Ovarian Neoplasms pathology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 29
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 17059009