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Characterization of immune responses elicited in macaques immunized sequentially with chimeric VEE/SIN alphavirus replicon particles expressing SIVGag and/or HIVEnv and with recombinant HIVgp140Env protein.
- Source :
-
AIDS research and human retroviruses [AIDS Res Hum Retroviruses] 2006 Oct; Vol. 22 (10), pp. 1022-30. - Publication Year :
- 2006
-
Abstract
- In the present study, macaques were coimmunized with VEErep/SINenv chimeric alphavirus replicon particles expressing SIVp55Gag and HIVDeltaV2gp140Env or only with replicon particles expressing HIVDeltaV2gp140Env. All animals were subsequently immunized with recombinant trimeric HIVDeltaV2gp140Env protein. During alphavirus immunization, anti-SIVGag and anti-HIVEnv-specific interferon (IFN)-gamma responses, as well as high titers of anti-HIVEnv binding (gp120 but not gp41 specific) and anti-HIV neutralizing antibodies, were generated. The subsequent immunization with recombinant HIVDeltaV2gp140 enhanced the neutralizing antibody titers and Env-specific IFN-gamma responses. Following intravenous challenge with the R5- tropic SHIV(SF162P4) virus, significantly lower primary plasma viremia levels were recorded in the immunized animals, as compared to control animals immunized with replicon particles expressing influenza virus HA. Our results show that this method of immunization elicits both strong cellular immunity and neutralizing antibodies in primates and, thus, merits further investigation.
- Subjects :
- Animals
Encephalitis Virus, Venezuelan Equine genetics
Genetic Vectors
Macaca mulatta
Recombinant Proteins immunology
Sindbis Virus
Vaccines, Synthetic immunology
env Gene Products, Human Immunodeficiency Virus
AIDS Vaccines immunology
Antibodies, Viral biosynthesis
Gene Products, env immunology
Gene Products, gag immunology
HIV Antibodies biosynthesis
Replicon
Subjects
Details
- Language :
- English
- ISSN :
- 0889-2229
- Volume :
- 22
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- AIDS research and human retroviruses
- Publication Type :
- Academic Journal
- Accession number :
- 17067273
- Full Text :
- https://doi.org/10.1089/aid.2006.22.1022