Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide.

Authors :: Marolda CL
Lahiry P
Vinés E
Saldías S
Valvano MA
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2006; Vol. 347, pp. 237-52.
Publication Year :: 2006
Abstract: Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.

Subjects :: Blotting, Western
Electrophoresis, Polyacrylamide Gel methods
Glycine analogs & derivatives
Glycine chemistry
Lipopolysaccharides analysis
Lipopolysaccharides chemistry
Molecular Probe Techniques
Silver Staining
Sugar Acids analysis
Sugar Acids chemistry
Sulfhydryl Compounds chemistry
Bacteriological Techniques methods
Lipid A analysis
O Antigens analysis

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