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In vitro effects of hydrochloric acid and various concentrations of acetic, propionic, butyric, or valeric acids on bioelectric properties of equine gastric squamous mucosa.
- Source :
-
American journal of veterinary research [Am J Vet Res] 2006 Nov; Vol. 67 (11), pp. 1873-82. - Publication Year :
- 2006
-
Abstract
- Objective: To compare the effects of hydrochloric acid (HCl) and various concentrations of volatile fatty acids (VFAs) on tissue bioelectric properties of equine stomach nonglandular (NG) mucosa.<br />Sample Population: Gastric tissues obtained from 48 adult horses.<br />Procedures: NG gastric mucosa was studied by use of Ussing chambers. Short-circuit current (Isc) and potential difference (PD) were measured and electrical resistance (R) and conductance calculated for tissues after addition of HCl and VFAs (5, 10, 20, and 40 mM) in normal Ringer's solution (NRS).<br />Results: Mucosa exposed to HCl in NRS (pH of 1.5 and, to a lesser extent, 4.0) had a significant decrease in Isc, PD, and R, whereas tissues exposed to acetic acid at a pH of < 4.0, propionic and butyric acids at a pH of <or= 4.0, and valeric acid at a pH of <or= 7.0 induced a concentration-dependent effect on reduction in these same values. Values for Isc returned to baseline (recovery of sodium transport) after addition of calcium carbonate in tissues exposed to all concentrations of VFAs except the higher concentrations of valeric acid at a pH of <or= 4.0. Histologic examination revealed cell swelling in the mucosal layers below and adjacent to the stratum corneum in tissues exposed to HCl and VFAs at a pH of <or= 4.0.<br />Conclusions and Clinical Relevance: The VFAs, especially acetic acid, in the presence of HCl at a pH of <or= 4.0 appear to be important in the pathogenesis of NG mucosal ulcers in horses.
- Subjects :
- Acetic Acid metabolism
Acetic Acid toxicity
Analysis of Variance
Animals
Butyric Acid metabolism
Butyric Acid toxicity
Dose-Response Relationship, Drug
Electric Conductivity
Electric Impedance
Fatty Acids, Volatile metabolism
Hydrochloric Acid metabolism
In Vitro Techniques
Membrane Potentials drug effects
Pentanoic Acids metabolism
Pentanoic Acids toxicity
Propionates metabolism
Propionates toxicity
Fatty Acids, Volatile toxicity
Gastric Mucosa drug effects
Horses metabolism
Hydrochloric Acid toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9645
- Volume :
- 67
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- American journal of veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 17078749
- Full Text :
- https://doi.org/10.2460/ajvr.67.11.1873