Back to Search
Start Over
Epigallocatechin gallate's protective effect against MMP7 in recessive dystrophic epidermolysis bullosa patients.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 2007 Apr; Vol. 127 (4), pp. 821-8. Date of Electronic Publication: 2006 Nov 30. - Publication Year :
- 2007
-
Abstract
- The analysis of phenotype-genotype correlations of patients suffering from recessive dystrophic epidermolysis bullosa (RDEB) evidenced intrafamilial and interfamilial phenotype variability occurring for the same mutation of COL7A1; this underscores the role of other genetics environmental factors in the expressivity of the disease. In this work, we checked whether matrilysin 1 (matrix metalloproteinase (MMP)7) could take part in the epidermal detachment in RDEB. Furthermore, we investigated epigallocatechin 3 gallate (EGCG) to determine whether it could inhibit matrilysin activities on collagen type VII and fibrillin 1 known to be associated with the dermo-epidermal junction. In this work, matrilysin 1 was detected in affected and unaffected skins of the three RDEB patients; furthermore, MMP7 was shown to degrade ex vivo on healthy normal skin collagen VII and fibrillin 1. Thus, we suspect that MMP7 could take an active part in the epidermal detachment occurring during RDEB. We evidenced that EGCG in in vitro as well as in ex vivo experiments was a good inhibitor of MMP7 and developed a good protection of collagen type VII and fibrillin 1 susceptible of being degraded by MMP7. We therefore propose that EGCG could be used beneficially in patients suffering from RDEB.
- Subjects :
- Adult
Antigens, CD metabolism
Antigens, Differentiation, Myelomonocytic metabolism
Blotting, Western
Catechin pharmacology
Collagen Type VII metabolism
Elastic Tissue pathology
Epidermolysis Bullosa Dystrophica genetics
Epidermolysis Bullosa Dystrophica pathology
Fibrillin-1
Fibrillins
Humans
Immunologic Techniques
Microfilament Proteins metabolism
Organ Culture Techniques
Skin enzymology
Skin pathology
Staining and Labeling
Time Factors
Tissue Distribution
Catechin analogs & derivatives
Epidermolysis Bullosa Dystrophica physiopathology
Genes, Recessive
Matrix Metalloproteinase 7 metabolism
Matrix Metalloproteinase Inhibitors
Protease Inhibitors pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1523-1747
- Volume :
- 127
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 17139264
- Full Text :
- https://doi.org/10.1038/sj.jid.5700645