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Detection of Tsh protein mucinolytic activity by SDS-PAGE.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2007 Mar; Vol. 68 (3), pp. 654-5. Date of Electronic Publication: 2006 Dec 08. - Publication Year :
- 2007
-
Abstract
- The temperature-sensitive hemagglutinin (Tsh, 140 kDa) produced by Escherichia coli is cleaved into a fragment (106 kDa) containing mucinase activity, and an agglutinin fragment (33 kDa). By incorporating mucins into SDS-PAGE gels stained by Schiff's periodic acid, we could simultaneously detect about 0.5 microg of mucinase activity and the fragment molecular mass.
Details
- Language :
- English
- ISSN :
- 0167-7012
- Volume :
- 68
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 17157399
- Full Text :
- https://doi.org/10.1016/j.mimet.2006.10.002