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A novel chimeric Plasmodium vivax circumsporozoite protein induces biologically functional antibodies that recognize both VK210 and VK247 sporozoites.
- Source :
-
Infection and immunity [Infect Immun] 2007 Mar; Vol. 75 (3), pp. 1177-85. Date of Electronic Publication: 2006 Dec 11. - Publication Year :
- 2007
-
Abstract
- A successful vaccine against Plasmodium vivax malaria would significantly improve the health and quality of the lives of more than 1 billion people around the world. A subunit vaccine is the only option in the absence of long-term culture of P. vivax parasites. The circumsporozoite protein that covers the surface of Plasmodium sporozoites is one of the best-studied malarial antigens and the most promising vaccine in clinical trials. We report here the development of a novel "immunologically optimal" recombinant vaccine expressed in Escherichia coli that encodes a chimeric CS protein encompassing repeats from the two major alleles, VK210 and VK247. This molecule is widely recognized by sera from patients naturally exposed to P. vivax infection and induces a highly potent immune response in genetically disparate strains of mice. Antibodies from immunized animals recognize both VK210 and VK247 sporozoites. Furthermore, these antibodies appear to be protective in nature since they cause the agglutination of live sporozoites, an in vitro surrogate of sporozoite infectivity. These results strongly suggest that recombinant CS is biologically active and highly immunogenic across major histocompatibility complex strains and raises the prospect that in humans this vaccine may induce protective immune responses.
- Subjects :
- Animals
Antibodies, Protozoan metabolism
Antibodies, Protozoan physiology
Female
Humans
Malaria Vaccines genetics
Malaria, Vivax immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Plasmodium vivax genetics
Protozoan Proteins genetics
Recombinant Fusion Proteins genetics
Sporozoites metabolism
Vaccines, Subunit genetics
Vaccines, Subunit immunology
Antibodies, Protozoan genetics
Malaria Vaccines immunology
Malaria, Vivax prevention & control
Plasmodium vivax immunology
Protozoan Proteins immunology
Recombinant Fusion Proteins immunology
Sporozoites immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 75
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 17158893
- Full Text :
- https://doi.org/10.1128/IAI.01667-06