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An RNA secondary structure juxtaposes two remote genetic signals for human T-cell leukemia virus type I RNA 3'-end processing.
- Source :
-
Journal of virology [J Virol] 1991 Oct; Vol. 65 (10), pp. 5165-73. - Publication Year :
- 1991
-
Abstract
- Sequence analysis of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) does not reveal a polyadenylation consensus sequence, AAUAAA, close to the polyadenylation site at the 3' end of the viral RNA. Using site-directed mutagenesis, we demonstrated that two cis-acting signals are required for efficient RNA processing in HTLV-I LTR: (i) a remote AAUAAA hexamer at a distance of 276 nucleotides upstream of the polyadenylation site, and (ii) the 20-nucleotide GU-rich sequence immediately downstream from the poly(A) site. It has been postulated that the folding of RNA into a secondary structure juxtaposes the AAUAAA sequence, in a noncontiguous manner, to within 14 nucleotides of the polyadenylation site. To test this hypothesis, we introduced deletions and point mutations within the U3 and R regions of the LTR. RNA 3'-end processing occurred efficiently at the authentic HTLV-I poly(A) site after deletion of the sequences predicted to form the secondary structure. Thus, the genetic analysis supports the hypothesis that folding of the HTLV-I RNA in the U3 and R regions juxtaposes the AAUAAA sequence and the poly(A) site to the correct functional distance. This unique arrangement of RNA-processing signals is also found in the related retroviruses HTLV-II and bovine leukemia virus.
- Subjects :
- Base Sequence
Cell Line
Chloramphenicol O-Acetyltransferase genetics
Chloramphenicol O-Acetyltransferase metabolism
Chromosome Deletion
Cloning, Molecular methods
DNA Probes
Genetic Vectors
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed
Nucleic Acid Conformation
Oligonucleotide Probes
Poly A genetics
Poly A isolation & purification
RNA genetics
RNA isolation & purification
RNA, Messenger
RNA, Viral chemistry
Retroviridae genetics
Transfection
Genes, Viral
Human T-lymphotropic virus 1 genetics
RNA, Viral genetics
Repetitive Sequences, Nucleic Acid
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 65
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 1716687
- Full Text :
- https://doi.org/10.1128/JVI.65.10.5165-5173.1991