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[Scanning aneugen and clastogen by micronuclei analysis using flow cytometry].
- Source :
-
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases [Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi] 2006 Nov; Vol. 24 (11), pp. 649-52. - Publication Year :
- 2006
-
Abstract
- Objective: To explore a flow cytometry (FCM)-based method for discriminating aneugen- or clastogen-induced micronuclei.<br />Methods: Cells were stained with anti-CD71-FITC and PI, and the PI fluorescent signal intensity of micronucleated reticulocyte (MN-RET) in the peripheral blood of NIH mouse treated with COL or CP was detected by flow cytometry.<br />Results: The ratio of the median of the intensity of MN-RET fluorescent signals to that of nucleated cell was low in the cyclophosphamide treated mouse, while the median was high in the colchicine treated mouse.<br />Conclusion: The flow cytometry-based micronucleus assay can be used to discriminate primarily smaller MN induced by the clastogen exposure from the larger MN induced by an aneugen.
Details
- Language :
- Chinese
- ISSN :
- 1001-9391
- Volume :
- 24
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
- Publication Type :
- Academic Journal
- Accession number :
- 17181940