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Expression of CCR9 in HTLV-1+ T cells and ATL cells expressing Tax.
- Source :
-
International journal of cancer [Int J Cancer] 2007 Apr 01; Vol. 120 (7), pp. 1591-7. - Publication Year :
- 2007
-
Abstract
- Adult T-cell leukemia (ATL) is a highly aggressive mature CD4+ T-cell malignancy that is etiologically associated with human T-lymphotropic virus Type 1 (HTLV-1). ATL is characterized by frequent infiltration of lymph nodes, spleen, liver, skin and gut. Previously, we and others have shown that the majority of ATL cases are strongly positive for CCR4, which may explain the frequent skin invasion of ATL. Here, we examined whether ATL cells express CCR9, which is involved in T-cell homing to the gastrointestinal tract. Human T cell lines carrying HTLV-1 consistently expressed CCR9 together with the HTLV-1-encoded transcriptional activator Tax. Although ATL cells freshly isolated from peripheral blood hardly expressed CCR9, ATL cells cultured for 1 day consistently expressed CCR9 in parallel with the upregulation of Tax. Induction of Tax by Cd2+ in JPX-9, a subline of Jurkat human T cell line carrying Tax under the control of metallothionein promoter, led to upregulation of CCR9. A luciferase reporter gene under the control of the CCR9 promoter was expressed by cotransfection of an expression vector for Tax or in Cd2+-treated JPX-9 cells. Furthermore, immunohistochemical staining demonstrated that ATL cells infiltrating gastrointestinal tract were frequently positive for CCR9. Collectively, CCR9 is inducible in ATL cells expressing Tax and may play a role in the gastrointestinal involvement of ATL.<br /> ((c) 2006 Wiley-Liss, Inc.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Cell Line, Transformed metabolism
Cell Line, Transformed pathology
Cell Line, Transformed virology
Female
Gastrointestinal Neoplasms pathology
Gene Products, tax genetics
Human T-lymphotropic virus 1 genetics
Humans
Leukemia-Lymphoma, Adult T-Cell pathology
Luciferases metabolism
Male
Middle Aged
Promoter Regions, Genetic genetics
Receptors, CCR
Receptors, CCR4
Receptors, Chemokine genetics
T-Lymphocytes pathology
T-Lymphocytes virology
Transcription, Genetic
Tumor Cells, Cultured metabolism
Tumor Cells, Cultured pathology
Tumor Cells, Cultured virology
Gastrointestinal Neoplasms metabolism
Gene Expression Regulation
Gene Products, tax metabolism
Leukemia-Lymphoma, Adult T-Cell metabolism
Receptors, Chemokine metabolism
T-Lymphocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 120
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 17205512
- Full Text :
- https://doi.org/10.1002/ijc.22483