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Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls.
- Source :
-
Animal reproduction science [Anim Reprod Sci] 2008 Jan 30; Vol. 103 (3-4), pp. 201-14. Date of Electronic Publication: 2006 Dec 13. - Publication Year :
- 2008
-
Abstract
- The performance of frozen-thawed spermatozoa from 10 Holstein bulls in a range of in vitro diagnostic tests and the relationship with adjusted in vivo fertility data was determined. The tests included an assessment of motility (subjective and computer-assisted), morphology, concentration, viability, acrosomal and chromatin integrity conducted immediately post-thaw and after swim-up, in conjunction with membrane status (CTC staining) and migration in an artificial cervical mucus. Adjusted in vivo fertility correlated with subjectively assessed post-thaw motility (r=0.672, p=0.033), post-thaw straight-line velocity (r=0.636, p=0.048), post-thaw sperm morphology (r=-0.762, p=0.010), post-thaw sperm viability (r=0.635, p=0.048), the concentration of spermatozoa after swim-up (r=0.649, p=0.042), sperm morphology after swim-up (r=-0.687, p=0.028), the number of spermatozoa migrating 10mm into artificial cervical mucus (r=0.632, p=0.050) and the distance migrated by the vanguard spermatozoon in artificial mucus (r=0.701, p=0.024). A stepwise regression analysis identified tests which, when combined, produced models with a strong correlation (R(2)>0.9) to fertility.
- Subjects :
- Acrosome physiology
Animals
Chromatin physiology
Dairying
Male
Regression Analysis
Sperm Capacitation physiology
Sperm Count veterinary
Sperm Motility physiology
Spermatozoa cytology
Cattle physiology
Cryopreservation veterinary
Fertility physiology
Semen Preservation veterinary
Spermatozoa physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0378-4320
- Volume :
- 103
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Animal reproduction science
- Publication Type :
- Academic Journal
- Accession number :
- 17208395
- Full Text :
- https://doi.org/10.1016/j.anireprosci.2006.12.010