[Determination of ginsenoside rd in human plasma by LC/MS/MS].

Authors :: Deng YH
Feng Y
Zeng X
Yang L
Liu YM
Huang Y
Sun J
Zhou D
Liang WX
Source :: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2006 Sep; Vol. 29 (9), pp. 928-31.
Publication Year :: 2006
Abstract: Objective: To develop a HPLC/MS/MS method for the determination of ginsenoside Rd in human plasma.<br />Methods: Plasma samples were pretreated by solid phase extraction (SPE). Ginsenoside Rd (m/z 964.6-->m/z 767.5) and gentiopicrin (m/z 374.1-->195.1) was detected by the positive electrospray ionization (ES+I)-MS method under multiple reaction monitoring (MRM) mode.<br />Results: The calibration curve of ginsenoside Rd in plasma was linear over the range of 3.00-5000.00 ng/ml. The limit of quantitation was 3.00 ng/ml. The relative recovery was 81.01-83.39%. The within-day and between-day RSDs were less than 15%. CONCLUSION This method is accurate, sensitive, and specific. It is suitable for the measurement of plasma ginsenoside Rd concentration.

Subjects :: Area Under Curve
Biological Availability
Drug Stability
Ginsenosides administration & dosage
Ginsenosides pharmacokinetics
Humans
Injections, Intravenous
Plants, Medicinal chemistry
Sensitivity and Specificity
Ginsenosides blood
Tandem Mass Spectrometry methods

Language :: Chinese
ISSN :: 1001-4454
Volume :: 29
Issue :: 9
Database :: MEDLINE
Journal :: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
Publication Type :: Academic Journal
Accession number :: 17212051

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