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[Determination of ginsenoside rd in human plasma by LC/MS/MS].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2006 Sep; Vol. 29 (9), pp. 928-31. - Publication Year :
- 2006
-
Abstract
- Objective: To develop a HPLC/MS/MS method for the determination of ginsenoside Rd in human plasma.<br />Methods: Plasma samples were pretreated by solid phase extraction (SPE). Ginsenoside Rd (m/z 964.6-->m/z 767.5) and gentiopicrin (m/z 374.1-->195.1) was detected by the positive electrospray ionization (ES+I)-MS method under multiple reaction monitoring (MRM) mode.<br />Results: The calibration curve of ginsenoside Rd in plasma was linear over the range of 3.00-5000.00 ng/ml. The limit of quantitation was 3.00 ng/ml. The relative recovery was 81.01-83.39%. The within-day and between-day RSDs were less than 15%. CONCLUSION This method is accurate, sensitive, and specific. It is suitable for the measurement of plasma ginsenoside Rd concentration.
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 29
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 17212051