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Isolation and characterization of the Serratia entomophila antifeeding prophage.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2007 May; Vol. 270 (1), pp. 42-8. Date of Electronic Publication: 2007 Jan 30. - Publication Year :
- 2007
-
Abstract
- The Serratia entomophila antifeeding prophage (Afp) is thought to form a virus-like structure that has activity towards the New Zealand grass grub, Costelytra zealandica. Through the trans based expression of AnfA1, an RfaH - like transcriptional antiterminator, the Afp, was able to be induced. The expressed Afp was purified and visualized by electron microscopy. The Afp resembled a phage tail-like bacteriocin, exhibiting two distinct morphologies: an extended and a contracted form. The purified Afp conferred rapid activity towards C. zealandica larvae, causing cessation of feeding and a change to an amber colouration within 48 h postinoculation, with increased dose rates causing larval mortality.
- Subjects :
- Animals
Arabinose pharmacology
Bacterial Proteins genetics
Bacterial Proteins pharmacology
Bacterial Proteins ultrastructure
DNA-Binding Proteins genetics
DNA-Binding Proteins pharmacology
DNA-Binding Proteins ultrastructure
Feeding Behavior drug effects
Gene Expression Regulation, Bacterial drug effects
Genes, Bacterial genetics
Larva microbiology
Microscopy, Electron, Transmission
Models, Biological
Polymerase Chain Reaction
Prophages genetics
Prophages ultrastructure
Serratia growth & development
Serratia virology
Temperature
Trans-Activators genetics
Trans-Activators pharmacology
Trans-Activators ultrastructure
Coleoptera microbiology
Prophages isolation & purification
Serratia genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 270
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 17263838
- Full Text :
- https://doi.org/10.1111/j.1574-6968.2007.00645.x