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The cellular proto-oncogene product Myb acts as transcriptional activator of the long terminal repeat of human T-lymphotropic virus type I.
- Source :
-
Journal of virology [J Virol] 1992 Jan; Vol. 66 (1), pp. 270-6. - Publication Year :
- 1992
-
Abstract
- The proto-oncogene c-myb encodes a nuclear transcription factor that binds to DNA in a sequence-specific manner and activates transcription of several viral and cellular genes. Expression of the c-myb gene is induced in mitogen- and/or antigen-stimulated T lymphocytes, which are also the preferential target cells of human T-lymphotropic virus type I (HTLV-I) in vivo and in vitro. We report here that Myb binds to the HTLV-I long terminal repeat (LTR) in four different regions in a sequence-specific manner. Electrophoretic mobility shift assay using labeled LTR fragments as well as labeled double-stranded oligonucleotides show that there are two high-affinity and two low-affinity Myb-binding sites present in the HTLV-I LTR. DNase I footprinting analysis and oligonucleotide competition experiments indicate that this binding is sequence specific. Cotransfection experiments in HeLa cells, using a Myb expression vector and chloramphenicol acetyltransferase reporter gene linked to the HTLV-I LTR, show that Myb activates HTLV-I LTR-mediated transcription by a factor of four-to sixfold. Thus, in HTLV-I-infected T cells, Myb protein binding to the HTLV-I LTR may constitute one of the signal that regulate HTLV-I transcription in vivo.
- Subjects :
- Base Sequence
Cloning, Molecular
DNA, Viral
Deoxyribonuclease I metabolism
Molecular Sequence Data
Oligonucleotides metabolism
Protein Binding
Proto-Oncogene Mas
Proto-Oncogene Proteins c-myb
Gene Expression Regulation, Viral
Human T-lymphotropic virus 1 genetics
Proto-Oncogene Proteins metabolism
Repetitive Sequences, Nucleic Acid
Trans-Activators metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 66
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 1727489
- Full Text :
- https://doi.org/10.1128/JVI.66.1.270-276.1992