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Analysis of CD8+ T cell-mediated anti-viral responses in mice with targeted deletions of the M1 or M5 muscarinic cholinergic receptors.

Authors :
Vezys V
Masopust D
Desmarets M
Wess J
Zimring JC
Source :
Life sciences [Life Sci] 2007 May 30; Vol. 80 (24-25), pp. 2330-3. Date of Electronic Publication: 2007 Jan 13.
Publication Year :
2007

Abstract

A number of studies have demonstrated that non-neuronal acetylcholine can play a role in the regulation of T cell function. Recently, we reported that CD8(+) T cells, from mice with a targeted deletion of the M(1) muscarinic receptor, had a defect in differentiating into cytolytic T lymphocytes when stimulated in vitro. In the current report, we analyze the in vivo function of CD8(+) T cells from mice with targeted deletions of either M(1) or M(5) muscarinic receptors. M(1) or M(5) knockout mice were infected with either lymphocytic choriomeningitis virus or vesicular stomatitis virus. Expansion of anti-viral CD8(+) T cells was monitored by staining with tetramer reagents specific for the immunodominant peptides of the viruses. No defect in expansion of CD8(+) T cells was observed in either M(1) or M(5) knockout mice. The extent to which one can draw a generalized conclusion that M(1) and M(5) are not involved in anti-viral immunity depends upon issues of antigen strength, genetic background, induction of redundant receptors, and the potential for qualitative defects in the expanded CD8(+) T cells.

Details

Language :
English
ISSN :
0024-3205
Volume :
80
Issue :
24-25
Database :
MEDLINE
Journal :
Life sciences
Publication Type :
Academic Journal
Accession number :
17286988
Full Text :
https://doi.org/10.1016/j.lfs.2007.01.001