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Hydrolysis of soybean isoflavonoid glycosides by Dalbergia beta-glucosidases.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2007 Mar 21; Vol. 55 (6), pp. 2407-12. Date of Electronic Publication: 2007 Feb 21. - Publication Year :
- 2007
-
Abstract
- Two beta-glucosidases from the legumes Dalbergia cochinchinensis and Dalbergia nigrescens were compared for their ability to hydrolyze isoflavonoid glycosides from soybean. Both D. nigrescens and D. cochinchinensis beta-glucosidases could hydrolyze conjugated soybean glycosides, but D. nigrescens beta-glucosidase hydrolyzed both conjugated and nonconjugated glycosides in crude soybean extract more rapidly. The kinetic properties Km, kcat, and kcat/Km of the Dalbergia beta-glucosidases toward conjugated isoflavonoid glycosides, determined using high-performance liquid chromatography, confirmed the higher efficiency of the D. nigrescens beta-glucosidase in hydrolyzing these substrates. The D. nigrescens beta-glucosidase could also efficiently hydrolyze isoflavone glycosides in soy flour suspensions, suggesting its application to increase free isoflavones in soy products.
Details
- Language :
- English
- ISSN :
- 0021-8561
- Volume :
- 55
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17311399
- Full Text :
- https://doi.org/10.1021/jf062885p