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Experimental infection of domestic cats with passaged genotype I Bartonella henselae.
Experimental infection of domestic cats with passaged genotype I Bartonella henselae.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2007 Jun 21; Vol. 122 (3-4), pp. 290-7. Date of Electronic Publication: 2007 Jan 31. - Publication Year :
- 2007
-
Abstract
- The influence of in vitro passage on Bartonella henselae pathogenesis in cats has not been thoroughly evaluated. Our objective was to examine the bacterial kinetics and humoral immune responses in cats experimentally infected with three different in vitro passages of B. henselae F1, a genotype I strain of feline origin. The F1 strain was in vitro passaged 20 and 40 times, and each was inoculated into a group of 5 cats. The kinetics of bacteremia and the feline humoral immune response to bacterial antigens were compared to a previous study involving a group of six cats inoculated with the original F1 strain. Among the three groups of cats, the kinetics of bacteremia profiles and the humoral immune responses to B. henselae lysates were similar. The influence of passage on bacterial membrane proteins was examined. In vitro passage altered the expression of 4/17 (23.5%) bacterial membrane proteins and 6/15 (40%) bacterial membrane antigens. An association between poor seroreactivity to three lysate antigens (15-, 18- and 45kDa), prolonged bacteremia and decreased serum bactericidal activity was noted. Our data show that in vitro passage of B. henselae did not alter the kinetics of bacteremia, including the occurrence of relapsing bacteremia, in experimentally infected cats. This suggests that highly passaged strains may not be suitable for future vaccination studies. Furthermore, in vitro passage results in phenotypic and antigenic changes in the bacterial membrane protein profile, which warrants caution in the interpretation of studies involving passaged B. henselae strains.
- Subjects :
- Animals
Antigens, Bacterial blood
Bacteremia epidemiology
Bacteremia veterinary
Bacterial Proteins chemistry
Bacterial Proteins immunology
Bacterial Vaccines immunology
Bartonella henselae growth & development
Blotting, Western veterinary
Cat Diseases prevention & control
Cat-Scratch Disease immunology
Cat-Scratch Disease microbiology
Cat-Scratch Disease prevention & control
Cats
Electrophoresis, Polyacrylamide Gel veterinary
Genotype
Kinetics
Neutralization Tests veterinary
Specific Pathogen-Free Organisms
Vaccines, Inactivated immunology
Antibody Formation
Bartonella henselae pathogenicity
Cat Diseases immunology
Cat Diseases microbiology
Cat-Scratch Disease veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1135
- Volume :
- 122
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 17321078
- Full Text :
- https://doi.org/10.1016/j.vetmic.2007.01.018