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Stable transformation and long-term maintenance of transgenic Taxus cell suspension cultures.

Stable transformation and long-term maintenance of transgenic Taxus cell suspension cultures.

Authors :
Ketchum RE
Wherland L
Croteau RB
Source :
Plant cell reports [Plant Cell Rep] 2007 Jul; Vol. 26 (7), pp. 1025-33. Date of Electronic Publication: 2007 Mar 01.
Publication Year :
2007

Abstract

A cell line of Taxus cuspidata has been transformed with wild-type Agrobacterium rhizogenes ATCC strain 15834 containing binary vector pCAMBIA1301 and, separately, with A. tumefaciens strain EHA105 containing binary vector pCAMBIA1305.2. Additionally, a cell line of T. chinensis has been transformed with wild-type A. rhizogenes ATCC strain 25818 containing binary vector pCAMBIA1301. The two transgenic T. cuspidata cell lines have been maintained in culture for more than 20 months, and the transgenic T. chinensis cell line for more than 9 months, with no loss of reporter gene expression or antibiotic resistance. The introduced genes had no discernable effect on growth or Taxol production in the transgenic cell lines when compared to the parent control. The methods for transforming non-embryogenic Taxus suspension cultures are described.

Details

Language :
English
ISSN :
0721-7714
Volume :
26
Issue :
7
Database :
MEDLINE
Journal :
Plant cell reports
Publication Type :
Academic Journal
Accession number :
17333018
Full Text :
https://doi.org/10.1007/s00299-007-0323-x