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Stable transformation and long-term maintenance of transgenic Taxus cell suspension cultures.
Stable transformation and long-term maintenance of transgenic Taxus cell suspension cultures.
- Source :
-
Plant cell reports [Plant Cell Rep] 2007 Jul; Vol. 26 (7), pp. 1025-33. Date of Electronic Publication: 2007 Mar 01. - Publication Year :
- 2007
-
Abstract
- A cell line of Taxus cuspidata has been transformed with wild-type Agrobacterium rhizogenes ATCC strain 15834 containing binary vector pCAMBIA1301 and, separately, with A. tumefaciens strain EHA105 containing binary vector pCAMBIA1305.2. Additionally, a cell line of T. chinensis has been transformed with wild-type A. rhizogenes ATCC strain 25818 containing binary vector pCAMBIA1301. The two transgenic T. cuspidata cell lines have been maintained in culture for more than 20 months, and the transgenic T. chinensis cell line for more than 9 months, with no loss of reporter gene expression or antibiotic resistance. The introduced genes had no discernable effect on growth or Taxol production in the transgenic cell lines when compared to the parent control. The methods for transforming non-embryogenic Taxus suspension cultures are described.
- Subjects :
- Cells, Cultured
Molecular Structure
Paclitaxel biosynthesis
Paclitaxel chemistry
Plant Proteins genetics
Plants, Genetically Modified
Sonication
Taxus drug effects
Taxus metabolism
Time Factors
Transformation, Genetic
Gene Expression Regulation, Plant physiology
Plant Proteins metabolism
Taxus cytology
Taxus genetics
Transgenes genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0721-7714
- Volume :
- 26
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 17333018
- Full Text :
- https://doi.org/10.1007/s00299-007-0323-x