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[The effect of serum high-density lipoprotein on the growth rate of human endothelial progenitor cells].
- Source :
-
Zhonghua bing li xue za zhi = Chinese journal of pathology [Zhonghua Bing Li Xue Za Zhi] 2006 Nov; Vol. 35 (11), pp. 672-6. - Publication Year :
- 2006
-
Abstract
- Objective: To study the effect of high-density lipoprotein (HDL) on the proliferation of endothelial progenitor cells (EPC) isolated from human umbilical cord blood; to further explore its effect on prevention and development of atherogenesis.<br />Methods: EPC isolated by density gradient centrifugation were cultured in a M200 medium. Immunofluorescence staining for CD133, CD34, KDR and Factor VIII were adopted respectively as the specific markers for identification. The effect of HDL on EPC proliferation was estimated on the 7th day of cell cultivation using MTT assay, confocal microscopy and fluorescence activated cell sorting.<br />Results: HDL, when incubated with EPC, was able to promote remarkably the proliferation rate of EPC, dose- and time-dependent. HDL participated in the transcriptional regulation of cell cycle by affecting the regulatory proteins such as cyclin D1.<br />Conclusions: A subtype of progenitor cells was isolated from human cord blood with a potential of differentiating into mature endothelial cells (known as endothelial progenitor cells). HDL plays an important role on EPC fluorescence activated cell sorting differentiation and proliferation. Further studies are required to identify the signal pathway and the molecular mechanism of HDL effect on EPC proliferation.
- Subjects :
- AC133 Antigen
Adult
Antigens, CD metabolism
Antigens, CD34 metabolism
Cell Cycle drug effects
Cell Differentiation drug effects
Cell Separation
Cells, Cultured
Cyclin D1 metabolism
Dose-Response Relationship, Drug
Endothelial Cells cytology
Endothelial Cells metabolism
Factor VIII metabolism
Fetal Blood cytology
Flow Cytometry
Fluorescent Antibody Technique
Glycoproteins metabolism
Humans
Lipoproteins, HDL blood
Microscopy, Confocal
Peptides metabolism
Stem Cells cytology
Stem Cells metabolism
Cell Proliferation drug effects
Endothelial Cells drug effects
Lipoproteins, HDL pharmacology
Stem Cells drug effects
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0529-5807
- Volume :
- 35
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Zhonghua bing li xue za zhi = Chinese journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 17374212