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[Detection of Mycobacterium tuberculosis DNA in sarcoidosis samples using real-time fluorescence polymerase chain reaction].
- Source :
-
Zhonghua bing li xue za zhi = Chinese journal of pathology [Zhonghua Bing Li Xue Za Zhi] 2006 Dec; Vol. 35 (12), pp. 735-7. - Publication Year :
- 2006
-
Abstract
- Objective: To study the role of Mycobacterium tuberculosis in the pathogenesis of sarcoidosis.<br />Methods: Archival material of 22 patients with a histologic diagnosis of sarcoidosis were retrieved. Real-time fluorescent polymerase chain reaction (PCR) was used to detect DNA fragments of the complex-specific insertion sequence IS6110 of Mycobacterium tuberculosis in formalin-fixed and paraffin-embedded biopsy samples.<br />Results: Among the 22 samples studied, Mycobacterium tuberculosis DNA was detected in 11 cases. The sequence of PCR amplified IS6110 DNA fragments completely matched with the related sequence in Mycobacterium tuberculosis gene.<br />Conclusions: Mycobacterium tuberculosis DNA is identified in a certain proportion of patients with a clinicopathologic diagnosis of sarcoidosis. Mycobacterium tuberculosis may be an important etiologic agent, at least in some of these patients.
- Subjects :
- Adult
Female
Fluorescence
Follow-Up Studies
Humans
Lymph Nodes microbiology
Lymph Nodes pathology
Male
Middle Aged
Mycobacterium tuberculosis genetics
Paraffin Embedding
Sarcoidosis pathology
DNA, Bacterial analysis
Mycobacterium tuberculosis isolation & purification
Polymerase Chain Reaction methods
Sarcoidosis microbiology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0529-5807
- Volume :
- 35
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Zhonghua bing li xue za zhi = Chinese journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 17374258