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Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan.
- Source :
-
Microbiology and immunology [Microbiol Immunol] 2007; Vol. 51 (3), pp. 271-7. - Publication Year :
- 2007
-
Abstract
- Pseudomonas aeruginosa showing resistance to imipenem were found in 100 of 1,058 strains (9.5%) from six hospitals (a-f) in Hiroshima City, Japan. Of the 100 strains, 14 (14%) were double disk synergy test positive using sodium mercaptoacetic acid disks, and 18 (18%) were bla(IMP-1) or bla(VIM-2) allele positive by polymerase chain reaction (PCR). Among 100 imipenem-resistant strains, 32 were categorized into multi-drug resistant strains, in which 13 were positive for the metallo-beta-lactamase gene. Fifty-one strains (51%) among the 100 imipenem-resistant strains had elevated RND efflux pump activity against levofloxacin. But only 6 of 51 strains were classified as multi-drug resistant strains. The pulsed field gel electrophoresis analysis of the Spe I-digested DNA from the 100 isolates suggested not only clonal spread but spread of heterogeneous clones started to contribute to the prevalence of metallo-beta-lactamase producing P. aeruginosa strains in Japanese hospitals.
- Subjects :
- Aged
Aged, 80 and over
Anti-Bacterial Agents pharmacology
Cross Infection microbiology
Drug Resistance, Multiple, Bacterial
Electrophoresis, Gel, Pulsed-Field
Female
Humans
Japan
Male
Multigene Family
Phylogeny
Pseudomonas aeruginosa isolation & purification
Pseudomonas aeruginosa metabolism
beta-Lactamases biosynthesis
beta-Lactamases genetics
Imipenem pharmacology
Pseudomonas Infections microbiology
Pseudomonas aeruginosa drug effects
Pseudomonas aeruginosa genetics
beta-Lactam Resistance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0385-5600
- Volume :
- 51
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17380046
- Full Text :
- https://doi.org/10.1111/j.1348-0421.2007.tb03908.x