Back to Search
Start Over
Identification of a novel septin 4 protein binding to human herpesvirus 8 kaposin A protein using a phage display cDNA library.
- Source :
-
Journal of virological methods [J Virol Methods] 2007 Jul; Vol. 143 (1), pp. 65-72. Date of Electronic Publication: 2007 Mar 23. - Publication Year :
- 2007
-
Abstract
- Human herpesvirus 8 (HHV-8) is associated with the development of Kaposi's sarcoma and several other human malignancies. Kaposin A protein of HHV-8 has been demonstrated as inducing tumorigenic transformation, being responsible for nuclear receptor coactivators in the transforming activity. In this study, a kaposin A-interacting septin 4 variant that contained the unique GDR at the N-terminus and AAALE at the C-terminus was identified using affinity selection of a phage display library. Co-immunoprecipitation and confocal imaging revealed in vitro binding specificity and in vivo co-localization of HHV-8 kaposin A protein to the septin 4 variant. The kaposin A-interacting septin 4 variant induced cell rounding up, activated caspase-3, and up-regulated transcriptional factor NF-kappaB. By contrast, kaposin A protein showed an antagonistic effect on the biological functions of the septin 4 variant. Therefore, the interaction of kaposin A protein and the septin 4 variant was suggested as playing a possible role in the development of HHV-8-associated malignancies. This study provides insights into the mechanism of the kaposin A protein pathology, in which the interactions of kaposin A protein with cellular proteins might allow alteration of fundamental cellular processes.
- Subjects :
- Apoptosis
Caspase 3 metabolism
Cell Line
Cytoskeletal Proteins antagonists & inhibitors
Enzyme Activation
GTP Phosphohydrolases antagonists & inhibitors
Humans
Microscopy, Confocal
NF-kappa B metabolism
Recombinant Proteins
Sarcoma, Kaposi
Septins
Signal Transduction
Up-Regulation
Cytoskeletal Proteins isolation & purification
Cytoskeletal Proteins metabolism
GTP Phosphohydrolases isolation & purification
GTP Phosphohydrolases metabolism
Herpesvirus 8, Human metabolism
Peptide Library
Viral Proteins isolation & purification
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0166-0934
- Volume :
- 143
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 17383018
- Full Text :
- https://doi.org/10.1016/j.jviromet.2007.02.010