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Biosynthesis of Wdr16, a marker protein for kinocilia-bearing cells, starts at the time of kinocilia formation in rat, and wdr16 gene knockdown causes hydrocephalus in zebrafish.
- Source :
-
Journal of neurochemistry [J Neurochem] 2007 Apr; Vol. 101 (1), pp. 274-88. - Publication Year :
- 2007
-
Abstract
- The rat ortholog of the WD40 repeat protein Wdr16 is abundantly expressed in testis and cultured ependymal cells. Low levels are found in lung and brain, respectively, while it is absent from kinocilia-free tissues. In testis and ependymal primary cultures, Wdr16 messenger RNA appears concomitantly with the messages for sperm-associated antigen 6, a kinocilia marker, and for hydin, a protein linked to ciliary function and hydrocephalus. In testis, ependyma and respiratory epithelium, the Wdr16 protein is up-regulated together with kinocilia formation. The wdr16 gene is restricted to genera in possession of kinocilia, and it is strongly conserved during evolution. The human and zebrafish proteins are identical in 62% of their aligned amino acids. On the message level, the zebrafish Wdr16 ortholog was found exclusively in kinocilia-bearing tissues by in situ hybridisation. Gene knockdown in zebrafish embryos by antisense morpholino injection resulted in severe hydrocephalus formation with unaltered ependymal morphology or ciliary beat. Wdr16 can be considered a differentiation marker of kinocilia-bearing cells. In the brain, it appears to be functionally related to water homeostasis or osmoregulation.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Biomarkers analysis
Biomarkers metabolism
Cells, Cultured
Ciliary Motility Disorders genetics
Ciliary Motility Disorders metabolism
Ciliary Motility Disorders physiopathology
Down-Regulation drug effects
Down-Regulation physiology
Ependyma abnormalities
Ependyma cytology
Ependyma metabolism
Evolution, Molecular
Humans
Hydrocephalus metabolism
Hydrocephalus physiopathology
Lateral Ventricles abnormalities
Lateral Ventricles metabolism
Lateral Ventricles physiopathology
Microfilament Proteins biosynthesis
Microfilament Proteins isolation & purification
Molecular Sequence Data
Nerve Tissue Proteins biosynthesis
Nerve Tissue Proteins isolation & purification
Oligonucleotides, Antisense pharmacology
Phylogeny
RNA, Messenger metabolism
Rats
Sequence Homology, Amino Acid
Water-Electrolyte Balance genetics
Water-Electrolyte Balance physiology
Zebrafish Proteins biosynthesis
Zebrafish Proteins isolation & purification
Cilia metabolism
Hydrocephalus genetics
Microfilament Proteins genetics
Nerve Tissue Proteins genetics
Zebrafish abnormalities
Zebrafish Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 101
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17394468
- Full Text :
- https://doi.org/10.1111/j.1471-4159.2007.04500.x