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Inhibition of anodic galvanotaxis of green paramecia by T-type calcium channel inhibitors.
- Source :
-
Zeitschrift fur Naturforschung. C, Journal of biosciences [Z Naturforsch C J Biosci] 2007 Jan-Feb; Vol. 62 (1-2), pp. 93-102. - Publication Year :
- 2007
-
Abstract
- Calcium ion (Ca2+) is one of the key regulatory elements for ciliary movements in the Paramecium species. It has long been known that members of Paramecium species including green paramecia (Paramecium bursaria) exhibit galvanotaxis which is the directed movement of cells toward the anode by swimming induced in response to an applied voltage. However, our knowledge on the mode of Ca2+ action during green paramecia anodic galvanotactic response is still largely limited. In the present study, quantification of anodic galvanotaxis was carried out in the presence and absence of various inhibitors of calcium signaling and calcium channels. Interestingly, galvanotactic movement of the cells was completely inhibited by a variety of Ca2+-related inhibitors. Such inhibitors include a Ca2+ chelator (EGTA), general calcium channel blockers (such as lanthanides), inhibitors of intracellular Ca2+ release (such as ruthenium red and neomycin), and inhibitors of T-type calcium channels (such as NNC 55-0396, 1-octanol and Ni2+). However, L-type calcium channel inhibitors such as nimodipine, nifedipine, verapamil, diltiazem and Cd2+ showed no inhibitory action. This may be the first implication for the involvement of T-type calcium channels in protozoan cellular movements.
- Subjects :
- Animals
Calcium Channel Blockers toxicity
Calcium Channels, T-Type drug effects
Cell Division drug effects
Cell Movement drug effects
Chelating Agents pharmacology
Chelating Agents toxicity
Electrophysiology
Neomycin pharmacology
Paramecium cytology
Paramecium drug effects
Ruthenium Red pharmacology
Thapsigargin pharmacology
Calcium Channel Blockers pharmacology
Calcium Channels, T-Type physiology
Cell Movement physiology
Paramecium physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0939-5075
- Volume :
- 62
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Zeitschrift fur Naturforschung. C, Journal of biosciences
- Publication Type :
- Academic Journal
- Accession number :
- 17425113
- Full Text :
- https://doi.org/10.1515/znc-2007-1-217