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Expression of CXC chemokine IP-10/Mob-1 by primary hepatocytes following heat shock.
- Source :
-
Saudi medical journal [Saudi Med J] 2007 Apr; Vol. 28 (4), pp. 514-8. - Publication Year :
- 2007
-
Abstract
- Objective: To examine the expression of chemokine IP-10/Mob-1 of hepatocytes in responses to the stress imposed during isolation by collagenase perfusion.<br />Methods: This study was performed in the Faculty of Life Sciences University of Manchester during 2001-2005. We employed western and northern blotting analysis to detect IP-10/Mob-1 in isolated and cultured hepatocytes in response to isolation stresses and under heat shock stimulation in this project.<br />Results: We showed that the ELR-CXC chemokine, IP-10/Mob-1 is secreted from isolated rat hepatocytes immediately after isolation and early during culture and IP-10/Mob-1, expression by hepatocytes was also stimulated in response to heat shock.<br />Conclusion: It seems that hepatocytes mimic the experiences of liver injury in vivo such as during stress, trauma, or after insults, and therefore, produce stress related agents like IP-10/Mob-1 chemokine to overcome such a injurious condition following isolation and heat shock stimulation. This study also provides a useful model to study the regulation of expression of this chemokines in vitro.
Details
- Language :
- English
- ISSN :
- 0379-5284
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Saudi medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 17457469