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A single dose of vitamin D enhances immunity to mycobacteria.
- Source :
-
American journal of respiratory and critical care medicine [Am J Respir Crit Care Med] 2007 Jul 15; Vol. 176 (2), pp. 208-13. Date of Electronic Publication: 2007 Apr 26. - Publication Year :
- 2007
-
Abstract
- Rationale: Vitamin D was used to treat tuberculosis (TB) in the preantibiotic era. Prospective studies to evaluate the effect of vitamin D supplementation on antimycobacterial immunity have not previously been performed.<br />Objectives: To determine the effect of vitamin D supplementation on antimycobacterial immunity and vitamin D status.<br />Methods: A double-blind randomized controlled trial was conducted in 192 healthy adult TB contacts in London, United Kingdom. Participants were randomized to receive a single oral dose of 2.5 mg vitamin D or placebo and followed up at 6 weeks.<br />Measurements and Main Results: The primary outcome measure was assessed with a functional whole blood assay (BCG-lux assay), which measures the ability of whole blood to restrict luminescence, and thus growth, of recombinant reporter mycobacteria in vitro; the readout is expressed as a luminescence ratio (luminescence postinfection/baseline luminescence). IFN-gamma responses to the Mycobacterium tuberculosis antigens early secretory antigenic target-6 and culture filtrate protein 10 were determined with a second whole blood assay. Vitamin D supplementation significantly enhanced the ability of participants' whole blood to restrict BCG-lux luminescence in vitro compared with placebo (mean luminescence ratio at follow-up, 0.57, vs. 0.71, respectively; 95% confidence interval for difference, 0.01-0.25; p=0.03) but did not affect antigen-stimulated IFN-gamma secretion.<br />Conclusions: A single oral dose of 2.5 mg vitamin D significantly enhanced the ability of participants' whole blood to restrict BCG-lux luminescence in vitro without affecting antigen-stimulated IFN-gamma responses. Clinical trials should be performed to determine whether vitamin D supplementation prevents reactivation of latent TB infection. Clinical trial registered with www.clinicaltrials.gov (NCT 00157066).
- Subjects :
- Adult
Aged
Aged, 80 and over
Double-Blind Method
Drug Administration Schedule
Female
Follow-Up Studies
Humans
Male
Middle Aged
Vitamin D administration & dosage
Vitamins administration & dosage
Dietary Supplements
Immunity, Innate drug effects
Tuberculosis immunology
Vitamin D pharmacology
Vitamins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1073-449X
- Volume :
- 176
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- American journal of respiratory and critical care medicine
- Publication Type :
- Academic Journal
- Accession number :
- 17463418
- Full Text :
- https://doi.org/10.1164/rccm.200701-007OC