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Studies on aminoisonucleoside modified siRNAs: stability and silencing activity.
- Source :
-
Bioconjugate chemistry [Bioconjug Chem] 2007 Jul-Aug; Vol. 18 (4), pp. 1017-24. Date of Electronic Publication: 2007 Jun 01. - Publication Year :
- 2007
-
Abstract
- A novel class of aminoisonucleoside was synthesized and incorporated into a luciferase gene-targeting siRNA. Structural and functional analyses of such a kind of siRNAs indicated that sense strand modifications with aminoisonucleoside at the 3' or 5' terminal, such as ssIso-1 and ssIso-2, have less effect on RNA duplex thermal and serum stabilities, and their functional activities are also comparable to their native siRNAs. In contrast, antisense strand modifications with aminoisonucleoside at the corresponding positions, such as asIso-2 or asIso-1, bring a striking negative effect on RNA duplex stability but still maintain around 40-50% of gene knockdown.
Details
- Language :
- English
- ISSN :
- 1043-1802
- Volume :
- 18
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Bioconjugate chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17539595
- Full Text :
- https://doi.org/10.1021/bc060398+