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[Construction of eukaryotic expression vector for VEGF165 gene and its expression in rat bladder smooth muscle cells].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2007 May; Vol. 27 (5), pp. 654-6. - Publication Year :
- 2007
-
Abstract
- Objective: To construct an eukaryotic expression vector for vascular endothelial growth factor (VEGF) 165 gene and obtain VEGF expression in rat bladder smooth muscle cells.<br />Methods: VEGF165 cDNA was cloned into the eukaryotic expression vector pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/VEGF165 was transfected into the rat bladder smooth muscle cells by electroporation. VEGF expression in the cells was determined by RT-PCR and immunofluoresence assay, and the biological activity of VEGF in the supernant of the transinfected cell culture was tested by MTT assay.<br />Results and Conclusion: VEGF expression was obtained in the transinfected cells, and the supernant of the transinfected cell cultures stimulated the proliferation of the endothelial cells.
- Subjects :
- Animals
Animals, Newborn
Cell Line
Cell Proliferation drug effects
Cells, Cultured
Cloning, Molecular
Culture Media, Conditioned metabolism
Culture Media, Conditioned pharmacology
Eukaryotic Cells metabolism
Fluorescent Antibody Technique
Gene Expression
Humans
Myocytes, Smooth Muscle cytology
Rats
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Urinary Bladder cytology
Vascular Endothelial Growth Factor A metabolism
Vascular Endothelial Growth Factor A pharmacology
DNA, Complementary genetics
Genetic Vectors genetics
Myocytes, Smooth Muscle metabolism
Vascular Endothelial Growth Factor A genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 27
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 17545081