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Cytokine profiling and in vitro studies of murine bone growth using biological fluids from children with juvenile idiopathic arthritis.
- Source :
-
Clinical endocrinology [Clin Endocrinol (Oxf)] 2007 Sep; Vol. 67 (3), pp. 442-8. Date of Electronic Publication: 2007 Jun 07. - Publication Year :
- 2007
-
Abstract
- Objective: Growth retardation in children with chronic inflammatory disease may be partly due to direct effects of pro-inflammatory cytokines on the growth plate and requires further investigation.<br />Design: This study assessed the cytokine concentrations in serum and synovial fluid (SF) in juvenile idiopathic arthritis (JIA), and determined the effect of the biological fluid on cultured murine metatarsal growth.<br />Patients: Serum and SF were obtained from four children attending for arthrocentesis (child A, systemic; children B, C and D, oligoarticular). In addition, serum samples were obtained from four more children (children E and F, polyarticular; child G, oligoarticular).<br />Measurements: Anthropometry, cytokine levels and longitudinal bone growth were assessed.<br />Results: Cytokines were elevated to a variable extent in the samples. Although all serum samples were associated with reduced metatarsal growth, only SF from child A and child B reduced metatarsal growth. Metatarsals treated with child A's SF showed reduced proliferation, reduced proliferative and mineralizing zone width, and increased hypertrophic zone width. Tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6 concentrations were elevated in child A's SF. However, SF exposure with neutralizing antibodies to these cytokines or IGF-1 did not improve metatarsal growth.<br />Conclusion: SF and serum JIA samples can impair bone growth at the growth plate. In synovial fluid, the effect is variable but resistant to treatment with IL-1beta, IL-6 and TNF-alpha specific antibodies and IGF-1, suggesting that other factors in this biological fluid may also have an effect on longitudinal growth through IGF-1-independent mechanisms.
- Subjects :
- Adolescent
Animals
Antibodies, Neutralizing pharmacology
Arthritis, Juvenile metabolism
Arthritis, Juvenile physiopathology
Blood Proteins pharmacology
Cell Division physiology
Child
Child, Preschool
Chondrocytes cytology
Chondrocytes immunology
Cytokines metabolism
Female
Growth Disorders metabolism
Growth Disorders physiopathology
Humans
Insulin-Like Growth Factor I metabolism
Male
Metatarsal Bones immunology
Metatarsal Bones metabolism
Mice
Organ Culture Techniques
Synovial Fluid metabolism
Arthritis, Juvenile immunology
Cytokines blood
Growth Disorders immunology
Metatarsal Bones growth & development
Synovial Fluid immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0300-0664
- Volume :
- 67
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Clinical endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 17555514
- Full Text :
- https://doi.org/10.1111/j.1365-2265.2007.02908.x