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Effects of cardiogenic edema fluid on ion and fluid transport in the adult lung.
- Source :
-
American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2007 Sep; Vol. 293 (3), pp. L651-9. Date of Electronic Publication: 2007 Jun 08. - Publication Year :
- 2007
-
Abstract
- We have previously shown that cardiogenic pulmonary edema fluid (EF) increases Na(+) and fluid transport by fetal distal lung epithelia (FDLE) (Rafii B, Gillie DJ, Sulowski C, Hannam V, Cheung T, Otulakowski G, Barker PM and O'Brodovich H. J Physiol 544: 537-548, 2002). We now report the effect of EF on Na(+) and fluid transport by the adult lung. We first studied primary cultures of adult type II (ATII) epithelium and found that overnight exposure to EF increased Na(+) transport, and this effect was mainly due to factors other than catecholamines. Plasma did not stimulate Na(+) transport in ATII. Purification of EF demonstrated that at least some agent(s) responsible for the amiloride-insensitive component resided within the globulin fraction. ATII exposed to globulins demonstrated a conversion of amiloride-sensitive short-circuit current (I(sc)) to amiloride-insensitive I(sc) with no increase in total I(sc). Patch-clamp studies showed that ATII exposed to EF for 18 h had increased the number of highly selective Na(+) channels in their apical membrane. In situ acute exposure to EF increased the open probability of Na(+)-permeant ion channels in ATII within rat lung slices. EF did increase, by amiloride-sensitive pathways, the alveolar fluid clearance from the lungs of adult rats. We conclude that cardiogenic EF increases Na(+) transport by adult lung epithelia in primary cell culture, in situ and in vivo.
- Subjects :
- Animals
Biological Transport
Blood Proteins metabolism
Catecholamines metabolism
Cell Membrane metabolism
Cell Polarity
Cells, Cultured
Epithelial Cells cytology
Epithelial Cells metabolism
Epithelial Sodium Channels metabolism
Ion Channel Gating
Ion Channels metabolism
Male
Pulmonary Alveoli metabolism
Rats
Rats, Sprague-Dawley
Sodium metabolism
Extravascular Lung Water metabolism
Lung metabolism
Pulmonary Edema metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1040-0605
- Volume :
- 293
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Lung cellular and molecular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 17557800
- Full Text :
- https://doi.org/10.1152/ajplung.00464.2006