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Identification of two polyphenolic compounds with antioxidant activities in longan pericarp tissues.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2007 Jul 11; Vol. 55 (14), pp. 5864-8. Date of Electronic Publication: 2007 Jun 19. - Publication Year :
- 2007
-
Abstract
- Longan fruits contain a significant amount of polyphenols. In the present study, polyphenols were extracted from longan pericarp tissues, and then two representative polyphenols were separated and purified by polyamide column chromatography, Sephadex LH-20 column chromatography, and silica gel column chromatography. On the basis of 1H NMR, 13C NMR, and electrospray ionization mass spectrometric (ESI-MS) data, the two compounds were identified as 4-O-methylgallic acid and (-)-epicatechin, respectively. In terms of reaction with longan polyphenol oxidase (PPO), (-)-epicatechin was further identified as the PPO substrate that caused longan fruit to brown. The results of antioxidant activity showed that 4-O-methylgallic acid had higher reducing power and 2,2-diphenyl-1-picrylhydrazyl- (DPPH-), hydroxyl radical-, and superoxide radical-scavenging activities than (-)-epicatechin.
- Subjects :
- Catechin isolation & purification
Catechin metabolism
Catechin pharmacology
Catechol Oxidase metabolism
Fruit chemistry
Gallic Acid analogs & derivatives
Gallic Acid isolation & purification
Gallic Acid metabolism
Gallic Acid pharmacology
Magnetic Resonance Spectroscopy
Polyphenols
Spectrometry, Mass, Electrospray Ionization
Antioxidants pharmacology
Flavonoids isolation & purification
Flavonoids pharmacology
Phenols isolation & purification
Phenols pharmacology
Sapindaceae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0021-8561
- Volume :
- 55
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17579438
- Full Text :
- https://doi.org/10.1021/jf070839z