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Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant mycobacterium tuberculosis strains.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2007 Sep; Vol. 45 (9), pp. 2802-7. Date of Electronic Publication: 2007 Jun 27. - Publication Year :
- 2007
-
Abstract
- Resistance of Mycobacterium tuberculosis to pyrazinamide (PZA) derives mainly from mutations in the pncA gene. We developed a reverse hybridization-based line probe assay with oligonucleotide probes designed to detect mutations in pncA. The detection of PZA resistance was evaluated in 258 clinical isolates of M. tuberculosis. The sensitivity and specificity of PZA resistance obtained by this new assay were both 100%, consistent with the results of conventional PZA susceptibility testing. This assay can be used with sputa from tuberculosis patients. It appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use.
- Subjects :
- Antitubercular Agents pharmacology
Humans
Mutation, Missense
Oligonucleotide Probes genetics
Sensitivity and Specificity
Sputum microbiology
Tuberculosis microbiology
Amidohydrolases genetics
Drug Resistance, Bacterial genetics
Microbial Sensitivity Tests methods
Mycobacterium tuberculosis genetics
Nucleic Acid Hybridization methods
Pyrazinamide pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 45
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 17596354
- Full Text :
- https://doi.org/10.1128/JCM.00352-07