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Internalization of MWCNTs by microglia: possible application in immunotherapy of brain tumors.
- Source :
-
NeuroImage [Neuroimage] 2007; Vol. 37 Suppl 1, pp. S9-17. Date of Electronic Publication: 2007 May 13. - Publication Year :
- 2007
-
Abstract
- There is a pressing need for new therapeutic, diagnostic, and drug delivery approaches for treating brain cancers. Nanotechnology offers a new method for targeted brain cancer therapy and could play a major role in gene and drug delivery. The goals of our study were to visualize in vitro ingestion, cytotoxicity, and loading capacity of Multi-Walled Carbon Nanotubes (MWCNTs) in microglia. Furthermore, we investigated internalization differences between microglia and glioma cells. BV2 microglia and GL261 glioma cells were incubated with MWCNTs, which were synthesized through catalytic chemical vapor deposition technique. Real-time RT-PCR, cell proliferation analysis, siRNA and DNA loading, electron microscopy, and flow cytometry were performed. We demonstrated that MWCNTs do not result in proliferative or cytokine changes in vitro, are capable of carrying DNA and siRNA and are internalized at higher levels in phagocytic cells as compared to tumor cells. This study suggests MWCNTs could be used as a novel, non-toxic, and biodegradable nano-vehicles for targeted therapy in brain cancers. Further studies are needed to demonstrate the full capacity of MWCNTs as nanovectors.
- Subjects :
- Animals
Cell Line, Tumor
Cell Proliferation drug effects
Cytokines metabolism
Drug Carriers
Flow Cytometry
Immunogenetics
Mice
Microscopy, Confocal
Microscopy, Electron, Scanning
Particle Size
Poloxamer
RNA
RNA, Small Interfering administration & dosage
RNA, Small Interfering pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Brain Neoplasms drug therapy
Immunotherapy methods
Microglia metabolism
Nanotubes
Subjects
Details
- Language :
- English
- ISSN :
- 1053-8119
- Volume :
- 37 Suppl 1
- Database :
- MEDLINE
- Journal :
- NeuroImage
- Publication Type :
- Academic Journal
- Accession number :
- 17601750
- Full Text :
- https://doi.org/10.1016/j.neuroimage.2007.03.078