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Functional characterization of two melanocortin (MC) receptors in lamprey showing orthology to the MC1 and MC4 receptor subtypes.
- Source :
-
BMC evolutionary biology [BMC Evol Biol] 2007 Jun 29; Vol. 7, pp. 101. Date of Electronic Publication: 2007 Jun 29. - Publication Year :
- 2007
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Abstract
- Background: The melanocortin (MC) receptors have a key role in regulating body weight and pigmentation. They belong to the rhodopsin family of G protein-coupled receptors (GPCRs). The purpose of this study was to identify ancestral MC receptors in agnathan, river lamprey.<br />Results: We report cloning of two MC receptors from river lamprey. The lamprey receptors, designated MCa and MCb, showed orthology to the MC1 and MC4 receptor subtypes, respectively. The molecular clock analysis suggested that lamprey MC receptor genes were not duplicated recently and diverged from each other more than 400 MYR ago. Expression and pharmacological characterization showed that the lamprey MCa receptor was able to bind and be activated by both lamprey and human MSH peptides. The lamprey MCa receptor had relatively high affinity for ACTH derived peptides similarly to the fish MC receptors. We found that both of the lamprey MC receptors were expressed in skin, while the MCb receptor was also found in liver, heart and skeletal muscle.<br />Conclusion: This study shows presence of MC receptors in agnathans indicating early signs of specific functions of melanocortin receptor subtypes.
- Subjects :
- Adrenocorticotropic Hormone metabolism
Amino Acid Sequence
Animals
Binding Sites
Binding, Competitive
Cell Line, Transformed
Cosyntropin metabolism
Cyclic AMP metabolism
Gene Duplication
Gene Library
Hagfishes genetics
Humans
Molecular Sequence Data
Organ Specificity
Peptide Fragments metabolism
Peptides, Cyclic metabolism
Phylogeny
Pro-Opiomelanocortin genetics
Protein Binding
Protein Interaction Mapping
Receptors, Melanocortin metabolism
Recombinant Fusion Proteins metabolism
Second Messenger Systems
Sequence Alignment
Sequence Homology, Amino Acid
Skin metabolism
Species Specificity
Viscera metabolism
alpha-MSH analogs & derivatives
alpha-MSH metabolism
beta-MSH metabolism
gamma-MSH metabolism
Evolution, Molecular
Petromyzon genetics
Receptor, Melanocortin, Type 1 genetics
Receptor, Melanocortin, Type 4 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2148
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- BMC evolutionary biology
- Publication Type :
- Academic Journal
- Accession number :
- 17603878
- Full Text :
- https://doi.org/10.1186/1471-2148-7-101