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Quantification of superparamagnetic iron oxide (SPIO)-labeled cells using MRI.
- Source :
-
Journal of magnetic resonance imaging : JMRI [J Magn Reson Imaging] 2007 Aug; Vol. 26 (2), pp. 366-74. - Publication Year :
- 2007
-
Abstract
- Purpose: To show the feasibility of using magnetic resonance imaging (MRI) to quantify superparamagnetic iron oxide (SPIO)-labeled cells.<br />Materials and Methods: Lymphocytes and 9L rat gliosarcoma cells were labeled with ferumoxides-protamine sulfate complex (FE-PRO). The cells were labeled efficiently (more than 95%) and the iron concentration inside each cell was measured by spectrophotometry (4.77-30.21 pg). Phantom tubes containing different numbers of labeled or unlabeled cells, as well as different concentrations of FE-PRO, were made. In addition, labeled and unlabeled cells were injected into fresh and fixed rat brains.<br />Results: Cellular viability and proliferation of labeled and unlabeled cells were shown to be similar. T2-weighted images were acquired using 7T and 3T MRI systems, and R2 maps of the tubes containing cells, free FE-PRO, and brains were made. There was a strong linear correlation between R2 values and labeled cell numbers, but the regression lines were different for the lymphocytes and gliosarcoma cells. Similarly, there was strong correlation between R2 values and free iron. However, free iron had higher R2 values than the labeled cells for the same concentration of iron.<br />Conclusion: Our data indicate that in vivo quantification of labeled cells can be done by careful consideration of different factors and specific control groups.<br /> ((c) 2007 Wiley-Liss, Inc.)
- Subjects :
- Animals
Brain anatomy & histology
Brain pathology
Cell Line, Tumor
Cell Proliferation
Cell Survival
Contrast Media pharmacology
Dextrans
Ferric Compounds metabolism
Ferrosoferric Oxide
Humans
Image Processing, Computer-Assisted
Iron pharmacology
Magnetite Nanoparticles
Oxides pharmacology
Phantoms, Imaging
Protamines pharmacology
Rats
Spectrophotometry
Ferric Compounds pharmacology
Magnetic Resonance Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1053-1807
- Volume :
- 26
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of magnetic resonance imaging : JMRI
- Publication Type :
- Academic Journal
- Accession number :
- 17623892
- Full Text :
- https://doi.org/10.1002/jmri.20978