Back to Search
Start Over
The effect of mineral trioxide aggregate on phagocytic activity and production of reactive oxygen, nitrogen species and arginase activity by M1 and M2 macrophages.
- Source :
-
International endodontic journal [Int Endod J] 2007 Aug; Vol. 40 (8), pp. 603-11. - Publication Year :
- 2007
-
Abstract
- Aim: To assess the influence of co-culture with mineral trioxide aggregate (MTA) on phagocytosis and the production of reactive oxygen intermediates (ROI) and nitrogen (NO) species and the arginase activity by M1 and M2 peritoneal macrophages.<br />Methodology: Cellular viability, adherence and phagocytosis of Saccharomyces boulardii were assayed in the presence of MTA. Macrophages were stimulated with zymosan for ROI assays and with Fusobacterium nucleatum and Peptostreptococcus anaerobius and IFN-gamma for NO production and arginase activity, when in contact with capillaries containing MTA. Data were analysed by T, anova, Kruskall-Wallis and Mann-Whitney tests.<br />Results: M2 macrophages displayed greater cellular viability in polypropylene tubes, greater ability to ingest yeast and smaller production of ROI and higher arginase activity when compared with M1 macrophages. Both macrophages, M1 and M2, presented similar cell adherence and NO production. The addition of bacterial preparations to macrophages interfered with NO and arginase productions. MTA did not interfere with any of the parameters measured.<br />Conclusions: Phagocytosis and the ability of the two macrophage subtypes to eliminate microbes were not affected by MTA.
- Subjects :
- Animals
Arginase drug effects
Drug Combinations
Female
Macrophages cytology
Macrophages drug effects
Male
Mice
Mice, Inbred C57BL
Reactive Nitrogen Species analysis
Reactive Oxygen Species analysis
Aluminum Compounds pharmacology
Calcium Compounds pharmacology
Oxides pharmacology
Phagocytosis drug effects
Root Canal Filling Materials pharmacology
Silicates pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0143-2885
- Volume :
- 40
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- International endodontic journal
- Publication Type :
- Academic Journal
- Accession number :
- 17627697
- Full Text :
- https://doi.org/10.1111/j.1365-2591.2007.01255.x