Back to Search
Start Over
Dihydroartemisinin exerts cytotoxic effects and inhibits hypoxia inducible factor-1alpha activation in C6 glioma cells.
- Source :
-
The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2007 Jun; Vol. 59 (6), pp. 849-56. - Publication Year :
- 2007
-
Abstract
- Artemisinin and its analogue dihydroartemisinin exert cytotoxic effects in some kinds of cancer cell lines. Here we determined whether dihydroartemisinin inhibits the growth and induces apoptosis of rat C6 glioma cells. We found dihydroartemisinin (5-25 microM) inhibited the growth and induced apoptosis of C6 cells in a concentration- and time-dependent manner; however, it was much less toxic to rat primary astrocytes. Dihydroartemisinin (5-25 microM) also increased the generation of reactive oxygen species in C6 cells. These effects of dihydroartemisinin were enhanced by ferrous ions (12.5-100 microM) and reduced by the iron chelator deferoxamine (25-200 microM). Immunoblotting analysis revealed that dihydroartemisinin (5-25 microM) significantly reduced hypoxia- and deferoxamine-induced expression of hypoxia inducible factor-1alpha and its target gene protein, vascular endothelial growth factor, in C6 cells. The results showed that dihydroartemisinin exerts a selective cytotoxic effect on C6 cells by increasing the reactive oxygen species and inhibiting hypoxia inducible factor-1alpha activation.
- Subjects :
- Animals
Antineoplastic Agents administration & dosage
Apoptosis
Artemisinins administration & dosage
Cell Hypoxia
Cell Proliferation drug effects
Cell Survival drug effects
Cells, Cultured
Chelating Agents pharmacology
Deferoxamine pharmacology
Ferrous Compounds pharmacology
Glioma
Hypoxia-Inducible Factor 1, alpha Subunit biosynthesis
Rats
Reactive Oxygen Species metabolism
Sesquiterpenes administration & dosage
Tumor Cells, Cultured
Vascular Endothelial Growth Factors biosynthesis
Antineoplastic Agents pharmacology
Artemisinins pharmacology
Hypoxia-Inducible Factor 1, alpha Subunit metabolism
Sesquiterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3573
- Volume :
- 59
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of pharmacy and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 17637177
- Full Text :
- https://doi.org/10.1211/jpp.59.6.0011