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Proteomic analysis of Schistosoma mansoni egg secretions.
- Source :
-
Molecular and biochemical parasitology [Mol Biochem Parasitol] 2007 Oct; Vol. 155 (2), pp. 84-93. Date of Electronic Publication: 2007 Jun 19. - Publication Year :
- 2007
-
Abstract
- Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced by egg antigens. In this study, using Multidimensional Protein Identification Technology, we identified the Schistosoma mansoni egg secretome consisting of 188 proteins. Notably we identified proteins involved in redox balance, molecular chaperoning and protein folding, development and signaling, scavenging and metabolic pathways, immune response modulation, and 32 novel, previously uncharacterized schistosome proteins. We localized a subset of previously characterized schistosome proteins identified in egg secretions in this study, to the surface of live S. mansoni eggs using the circumoval precipitin reaction. The identification of proteins actively secreted by live schistosome eggs provides important new information for understanding immune modulation and the pathology of schistosomiasis.
- Subjects :
- Amino Acid Sequence
Animals
Antigens, Helminth analysis
Chromatography, High Pressure Liquid
Host-Parasite Interactions
Mass Spectrometry
Membrane Proteins analysis
Models, Molecular
Molecular Sequence Data
Sequence Alignment
Helminth Proteins analysis
Ovum chemistry
Proteome analysis
Schistosoma mansoni chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0166-6851
- Volume :
- 155
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular and biochemical parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 17644200
- Full Text :
- https://doi.org/10.1016/j.molbiopara.2007.06.002