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Characterization of the Erwinia chrysanthemi Gan locus, involved in galactan catabolism.
- Source :
-
Journal of bacteriology [J Bacteriol] 2007 Oct; Vol. 189 (19), pp. 7053-61. Date of Electronic Publication: 2007 Jul 20. - Publication Year :
- 2007
-
Abstract
- beta-1,4-Galactan is a major component of the ramified regions of pectin. Analysis of the genome of the plant pathogenic bacteria Erwinia chrysanthemi revealed the presence of a cluster of eight genes encoding proteins potentially involved in galactan utilization. The predicted transport system would comprise a specific porin GanL and an ABC transporter made of four proteins, GanFGK(2). Degradation of galactans would be catalyzed by the periplasmic 1,4-beta-endogalactanase GanA, which released oligogalactans from trimer to hexamer. After their transport through the inner membrane, oligogalactans would be degraded into galactose by the cytoplasmic 1,4-beta-exogalactanase GanB. Mutants affected for the porin or endogalactanase were unable to grow on galactans, but they grew on galactose and on a mixture of galactotriose, galactotetraose, galactopentaose, and galactohexaose. Mutants affected for the periplasmic galactan binding protein, the transporter ATPase, or the exogalactanase were only able to grow on galactose. Thus, the phenotypes of these mutants confirmed the functionality of the gan locus in transport and catabolism of galactans. These mutations did not affect the virulence of E. chrysanthemi on chicory leaves, potato tubers, or Saintpaulia ionantha, suggesting an accessory role of galactan utilization in the bacterial pathogeny.
- Subjects :
- ATP-Binding Cassette Transporters genetics
ATP-Binding Cassette Transporters metabolism
Bacterial Proteins chemistry
Bacterial Proteins genetics
Bacterial Proteins metabolism
Cichorium intybus microbiology
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Bacterial drug effects
Genes, Bacterial
Genome, Bacterial
Glucose pharmacology
Multigene Family
Mutation
Protons
Solanum tuberosum microbiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sucrose pharmacology
Temperature
Dickeya chrysanthemi genetics
Dickeya chrysanthemi metabolism
Galactans metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 189
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 17644603
- Full Text :
- https://doi.org/10.1128/JB.00845-07