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[Molecular genetic approaches in parasitology (in case of opisthorchiasis)].
- Source :
-
Meditsinskaia parazitologiia i parazitarnye bolezni [Med Parazitol (Mosk)] 2007 Apr-Jun (2), pp. 3-6. - Publication Year :
- 2007
-
Abstract
- Light, electron microscopy, polymerase chain reaction (PCR), and immunohistochemistry were used to reveal the proliferative, alterative processes of hepatic parenchymal cells, P 53, K-ras, B-raf, c-Kit, p 16, APC, immunoreactive proteins Ki-67, cytokeratins 9, 14, 20, EMA, c-erb B-2, CD-117, mutated p 53 and bcl-2 genes. P53, K-ras, B-raf, c-Kit, and p 16 gene mutations were detected in the liver of animals with experimental superinvasive opisthorchiasis (SO) and cholangiocellular carcinoma (CCC). Mutations of these genes were found in the plasma samples taken from SO patients. The CCC tissue from SO patients displayed expression of the following genes: p 53 (100.0%), p 53+B-raf (50.0%), K-ras+c-Kit (33.3%), K-ras+B-rafat more than 5 cm from the tumor in 2 cases. Following 2 years, target therapy (canglait, megamin, immunomodulators) leveled mutations in 91.25% of the patients with SO.
- Subjects :
- Animals
Bile Duct Neoplasms etiology
Bile Duct Neoplasms metabolism
Bile Duct Neoplasms pathology
Cholangiocarcinoma etiology
Cholangiocarcinoma metabolism
Cholangiocarcinoma pathology
Cricetinae
DNA-Binding Proteins genetics
Genes, ras genetics
Helix-Turn-Helix Motifs genetics
Hepatocytes metabolism
Humans
Immunologic Factors therapeutic use
Liver metabolism
Liver pathology
Male
Molecular Biology
Mutation drug effects
Opisthorchiasis complications
Proto-Oncogene Proteins B-raf genetics
Proto-Oncogene Proteins c-kit genetics
Zeolites metabolism
Hepatocytes pathology
Opisthorchiasis genetics
Opisthorchiasis therapy
Opisthorchis
Subjects
Details
- Language :
- Russian
- ISSN :
- 0025-8326
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Meditsinskaia parazitologiia i parazitarnye bolezni
- Publication Type :
- Academic Journal
- Accession number :
- 17663039