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[Protective effect of verapamil pretreatment against cerebral ischemia-reperfusion injury in gerbils].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2007 Jul; Vol. 27 (7), pp. 1061-4. - Publication Year :
- 2007
-
Abstract
- Objective: To observe the protective effect of verapamil pretreatment against cerebral ischemia-reperfusion injury in gerbils.<br />Methods: Thirty-three Mongolian gerbils were randomized into the control group (group A, n=6, with sham operation), ischemia group (group B), and 3 verapamil groups (groups C, D, and E, n=7) with intraperitpneal verapamil injection (2 mg/kg) 48, 24 and 12 h before ischemia, respectively. In group A, the bilateral common carotid arteries were only exposed without clamping, and in the other 4 groups, the arteries were clamped for 20 min followed by reperfusion for 50 min. The gerbils were then decapitated and the forebrain cerebral cortex was removed to determine superoxide dismutase (SOD) and glutathione (GSH) activities and measure the contents of malondial dehyde (MDA), endothelin (ET) and calcitonin gene-related peptide (CGRP). The left forebrain cerebral cortex was sampled in each group to observe the ultrastructural changes under electron microscope.<br />Results: In groups C and D, SOD activities were significantly higher than those in group B (P<0.05), and in group E, the SOD activity elevation was not statistically significant (P>0.05). In groups C, D and E, GSH activity was significantly higher than that in group B (P<0.05). MDA content was significantly lower in groups C and D than in group B (P<0.05), but comparable between groups E and B (P>0.05). ET content was also significantly lower in the pretreatment groups (P<0.05), but CGRP content higher (not statistically so, however) than those in group B. The more serious ultrastructural damage of the cerebral tissue was observed in group B, but only mild damage was found in the verapamil groups.<br />Conclusions: Verapamil given 12-48 h before cerebral ischemia may protect the gerbils from cerebral ischemia-reperfusion injury by enhancing SOD, GSH activities and decreasing ET content.
- Subjects :
- Animals
Brain drug effects
Brain metabolism
Brain pathology
Brain Ischemia metabolism
Brain Ischemia pathology
Endothelins metabolism
Gerbillinae
Glutathione metabolism
Malondialdehyde metabolism
Receptors, Calcitonin Gene-Related Peptide metabolism
Reperfusion Injury metabolism
Reperfusion Injury pathology
Superoxide Dismutase metabolism
Brain Ischemia complications
Brain Ischemia prevention & control
Reperfusion Injury complications
Reperfusion Injury prevention & control
Verapamil pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 27
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 17666350