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MHC class II+ exosomes in plasma suppress inflammation in an antigen-specific and Fas ligand/Fas-dependent manner.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2007 Aug 15; Vol. 179 (4), pp. 2235-41. - Publication Year :
- 2007
-
Abstract
- Exosomes are 50- to 100-nm vesicles that are formed within the late endocytic compartment and released from a variety of cell types. Previously, we demonstrated that exosomes derived from dendritic cells transduced with adenoviral vectors expressing IL-10, IL-4, or Fas ligand (FasL) produce anti-inflammatory exosomes able to reduce inflammation in a murine paw delayed-type hypersensitivity model, suppress the onset on murine collagen-induced arthritis, and reduce the severity of established collagen-induce arthritis. In this study, we examined the ability of endogenous, blood-borne exosomes to regulate the immune response. Exosomes isolated from plasma of mice immunized to keyhole limpet hemocyanin, but not from naive or OVA-immunized mice, were able to suppress the keyhole limpet hemocyanin-specific delayed-type hypersensitivity inflammatory response. The anti-inflammatory effect was mediated by MHC class II(+) plasma exosomes that were also FasL(+) and CD11b(+), but CD11c(-). Moreover, the anti-inflammatory effect of the MHC class II(+) plasma-derived exosomes was, in part, dependent upon the presence of FasL in the exosomes and Fas in the recipient mouse. These results suggest that exosomes in the plasma, produced by MHC class II(+) and CD11b(+) cells, have the ability to suppress the immune response in an Ag-specific manner in part through a Fas/FasL-dependent manner.
- Subjects :
- Adenoviridae
Animals
Antigens, CD1 genetics
Antigens, CD1 immunology
Arthritis, Experimental genetics
Arthritis, Experimental immunology
Dendritic Cells immunology
Hemocyanins immunology
Hemocyanins pharmacology
Hypersensitivity, Delayed immunology
Inflammation genetics
Inflammation immunology
Interleukin-10 immunology
Interleukin-4 immunology
Male
Mice
Mice, Inbred MRL lpr
Ovalbumin immunology
Ovalbumin pharmacology
Transduction, Genetic
Transport Vesicles genetics
Antigens immunology
Fas Ligand Protein immunology
Histocompatibility Antigens Class II immunology
Immune Tolerance genetics
Plasma immunology
Transport Vesicles immunology
fas Receptor immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 179
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 17675484
- Full Text :
- https://doi.org/10.4049/jimmunol.179.4.2235