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Microbiologic contamination of intraoperative blood salvaged during liver transplantation.
- Source :
-
Transplantation proceedings [Transplant Proc] 2007 Jul-Aug; Vol. 39 (6), pp. 1889-91. - Publication Year :
- 2007
-
Abstract
- Bacterial contamination is one of the potential risks of blood salvage and reinfusion during orthotopic liver transplantation (OLT) because cell-saver machines lack antibacterial protection devices. This study was designed to analyze the potential bacterial contamination of blood salvaged during OLT; a secondary end point was to evaluate whether reinfusion of potentially contaminated blood may have been responsible for clinically manifested infective complications in the same patient. After induction of anesthesia, a blood sample was drawn from the central venous catheter (CVC) immediately after its positioning, to exclude potential coexisting hematic contamination of the recipient. During the procedure, 2 other samples of salvaged blood were collected for bacteriological analysis. Twenty-six of 38 samples of salvaged blood were positive for microorganisms, whereas 12 did not reveal the presence of infectious agents. In 19 of 26 positive samples, Staphylococcus species (73%) were isolated with only 2 of 38 samples drawn from CVC being contaminated. Candida Albicans was cultured in 2 samples. The high percentage (73%) of coagulase-negative Staphylococci indicates that blood contamination could have been caused by microorganisms from the air or suctioned from contact surfaces and the surgical field. Although almost 70% of processed and reinfused units tested positive for microbes, none of the postoperative blood cultures (at day 1 and day 3) revealed growth of the same species, not even in the 2 patients who had positive CVC cultures after induction of anesthesia.
- Subjects :
- Adolescent
Adult
Aged
Blood Transfusion, Autologous
Candida albicans isolation & purification
Equipment Contamination
Escherichia coli isolation & purification
Female
Humans
Male
Middle Aged
Reoperation
Staphylococcus isolation & purification
Transfusion Reaction
Blood Loss, Surgical
Intraoperative Period
Liver Transplantation adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 0041-1345
- Volume :
- 39
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Transplantation proceedings
- Publication Type :
- Academic Journal
- Accession number :
- 17692644
- Full Text :
- https://doi.org/10.1016/j.transproceed.2007.05.005