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The combination of PCR and serology increases the diagnosis of Pseudomonas aeruginosa colonization/infection in cystic fibrosis.
- Source :
-
Pediatric pulmonology [Pediatr Pulmonol] 2007 Oct; Vol. 42 (10), pp. 938-44. - Publication Year :
- 2007
-
Abstract
- Background: Early diagnosis of Pseudomonas aeruginosa colonization/infection in patients with cystic fibrosis (CF) using microbiological culturing methods may be difficult. Serology and polymerase chain reaction (PCR) may be useful techniques for early detection of P. aeruginosa in children with CF.<br />Methods: A cross-sectional analysis comparing results obtained by three different methods for P. aeruginosa identification was performed in 87 CF patients with a mean age of 9.7 years. Microbiological culturing and PCR targeting the algD GDP mannose dehydrogenase gene of P. aeruginosa were performed in sputum or oropharyngeal swabs samples, and serum antibodies against three P. aeruginosa antigens (elastase, alkaline protease, and exotoxin A) were assessed once.<br />Results: It was possible to isolate P. aeruginosa by culture in samples from 42 patients (48.2%), while PCR was positive in 53 (60.9%) patients. Serology was positive in 38 patients (43.6%), with a higher positivity for elastase (37.9%), followed by alkaline protease (29.9%) and exotoxin A (19.5%). The difference among the three isolated methods was not statistically significant. The combination of PCR + serology was significantly superior to single methods, to PCR + culture and also to culture + serology.<br />Conclusions: PCR identified a higher number of patients with P. aeruginosa than serology and conventional culture, but the difference did not reach statistical significance. Any of the combination methods that included PCR resulted in significantly statistical differences in relation to isolated microbiological or serology methods, but not to the PCR method alone, suggesting that PCR may be the main additive method for P. aeruginosa identification.<br /> ((c) 2007 Wiley-Liss, Inc.)
- Subjects :
- Adolescent
Adult
Antibodies, Bacterial blood
Bacteriological Techniques
Child
Child, Preschool
Cross-Sectional Studies
Cystic Fibrosis complications
Female
Humans
Infant
Male
Pseudomonas Infections complications
Serologic Tests
Cystic Fibrosis microbiology
Polymerase Chain Reaction
Pseudomonas Infections diagnosis
Pseudomonas aeruginosa isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 8755-6863
- Volume :
- 42
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Pediatric pulmonology
- Publication Type :
- Academic Journal
- Accession number :
- 17722007
- Full Text :
- https://doi.org/10.1002/ppul.20686