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Involvement of Escherichia coli in pathogenesis of xanthogranulomatous cholecystitis with scavenger receptor class A and CXCL16-CXCR6 interaction.
- Source :
-
Pathology international [Pathol Int] 2007 Oct; Vol. 57 (10), pp. 652-63. - Publication Year :
- 2007
-
Abstract
- Xanthogranulomatous cholecystitis (XGC) is characterized by the infiltration of numerous foamy macrophages. Bacterial infection is thought to be involved in the pathogenesis of XGC. Using XGC and cultured murine biliary epithelial cells (BEC), the participation of E. coli and the role of the scavenger receptor class A (SCARA), as well as chemokine(C-X-C motif) ligand 16 (CXCL16) and its receptor chemokine(C-X-C motif) receptor 6 (CXCR6), were examined in the pathogenesis of XGC. E. coli components and genes were detected in XGC on immunohistochemistry and polymerase chain reaction (PCR), respectively. SCARA-recognizing E. coli was found in foamy macrophages aggregated in xanthogranulomatous lesions. CXCL16, which functions as a membrane-bound molecule and soluble chemokine to induce adhesion and migration of CXCR6(+) cells, was detected on gallbladder epithelia, and CXCR6(+)/CD8(+) T cells and CXCR6(+)/CD68(+) macrophages were also accumulated. In cultured BEC, CXCL16 mRNA and secreted soluble CXCL16 were constantly detected and upregulated by treatment with E. coli and lipopolysaccharide through Toll-like receptor 4. These suggest that SCARA in macrophages is involved in the phagocytosis of E. coli followed by foamy changes and that bacterial infection causes the upregulation of CXCL16 in gallbladder epithelia, leading to the chemoattraction of macrophages via CXCL16-CXCR6 interaction and formation of the characteristic histology of XGC.
- Subjects :
- Aged
Aged, 80 and over
Animals
Bile Ducts, Intrahepatic cytology
CD8-Positive T-Lymphocytes metabolism
CD8-Positive T-Lymphocytes pathology
Cells, Cultured
Chemokine CXCL16
Cholecystitis metabolism
Cholecystitis pathology
Epithelial Cells cytology
Epithelial Cells metabolism
Escherichia coli genetics
Escherichia coli isolation & purification
Female
Foam Cells metabolism
Foam Cells microbiology
Genes, Bacterial genetics
Granuloma metabolism
Granuloma pathology
Humans
Immunohistochemistry
Male
Mice
Middle Aged
Phagocytosis
Receptors, CXCR6
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
Xanthomatosis metabolism
Xanthomatosis pathology
Chemokines, CXC metabolism
Cholecystitis microbiology
Escherichia coli pathogenicity
Granuloma microbiology
Receptors, Chemokine metabolism
Receptors, Scavenger metabolism
Receptors, Virus metabolism
Scavenger Receptors, Class A metabolism
Xanthomatosis microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1320-5463
- Volume :
- 57
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Pathology international
- Publication Type :
- Academic Journal
- Accession number :
- 17803654
- Full Text :
- https://doi.org/10.1111/j.1440-1827.2007.02154.x