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Can ID repetitive elements serve as cis-acting dendritic targeting elements? An in vivo study.
- Source :
-
PloS one [PLoS One] 2007 Sep 26; Vol. 2 (9), pp. e961. Date of Electronic Publication: 2007 Sep 26. - Publication Year :
- 2007
-
Abstract
- Dendritic localization of mRNA/RNA involves interaction of cis-elements and trans-factors. Small, non-protein coding dendritic BC1 RNA is thought to regulate translation in dendritic microdomains. Following microinjections into cultured cells, BC1 RNA fused to larger mRNAs appeared to impart transport competence to these chimeras, and its 5' ID region was proposed as the cis-acting dendritic targeting element. As these ID elements move around rodent genomes and, if transcribed, form a long RNA stem-loop, they might, thereby, lead to new localizations for targeted gene products. To test their targeting ability in vivo we created transgenic mice expressing various ID elements fused to the 3' UTR of reporter mRNA for Enhanced Green Fluorescent Protein. In vivo, neither ID elements nor the BC1 RNA coding region were capable of transporting EGFP RNA to dendrites, although the 3' UTR of alpha-CaMKII mRNA, an established cis-acting element did produce positive results. Other mRNAs containing naturally inserted ID elements are also not found in neuronal dendrites. We conclude that the 5' ID domain from BC1 RNA is not a sufficient dendritic targeting element for mRNAs in vivo.
- Subjects :
- 3' Untranslated Regions genetics
Animals
Blotting, Northern
Blotting, Southwestern
Calcium-Calmodulin-Dependent Protein Kinase Type 2 genetics
Calcium-Calmodulin-Dependent Protein Kinase Type 2 metabolism
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Hippocampus metabolism
In Situ Hybridization
Mice
Mice, Transgenic
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Small Cytoplasmic genetics
RNA, Small Cytoplasmic metabolism
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Brain metabolism
Dendrites metabolism
Repetitive Sequences, Nucleic Acid genetics
Short Interspersed Nucleotide Elements genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 2
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 17896001
- Full Text :
- https://doi.org/10.1371/journal.pone.0000961